Moretto Thais Ludmilla, Benfato Izabelle Dias, de Carvalho Francine Pereira, Barthichoto Marcela, Le Sueur-Maluf Luciana, de Oliveira Camila Aparecida Machado
Departament of Biosciences, Federal University of Sao Paulo, Campus Baixada Santista; R. Silva Jardim 136, Vila Mathias, 11015-020 Santos, SP, Brazil.
Departament of Biosciences, Federal University of Sao Paulo, Campus Baixada Santista; R. Silva Jardim 136, Vila Mathias, 11015-020 Santos, SP, Brazil.
Life Sci. 2017 Jun 15;179:30-36. doi: 10.1016/j.lfs.2017.04.017. Epub 2017 Apr 25.
To characterize the effects of a calorie matched high-fat diet (HFD) on spontaneous physical activity (SPA), body weight, inflammatory status and expression of genes related to energy homeostasis in hypothalamus of mice.
C57Bl/6 mice (n=5 per group) were fed a control diet (16.5% calories from fat) - control group (C), or a calorie matched HFD (60% calories from fat). We evaluated, periodically, body weight and SPA by infrared beam sensors and, at the end of the 12th week, we verified blood glucose levels, fat pads weight, plasma insulin, TNF-α and IL-6 by ELISA and the hypothalamic expression of 84 genes related to energy homeostasis, by quantitative real-time PCR array.
Isocaloric HFD reduced SPA already in the first 48h and SPA was kept lower in the HFD compared to C throughout. These changes resulted in an increase in body weight, adiposity, TNF-α and IL-6, blood glucose and hyperinsulinemia in the HFD group when compared to the C group. Expression of the Agrp, Bdnf, Adra2b and Pyy genes were altered in the hypothalamus of HFD-fed mice, highlighting the downregulation of Bdnf, key regulator of energy homeostasis.
Dietary macronutrient distribution plays an important part in energy homeostasis that goes beyond its energy content. Despite calorie-matched, the HFD led to increased body weight and adiposity due to decreased SPA, highlighting the key role of SPA on energy balance. The changes in hypothalamic gene expression seem to underlie the reduction in SPA caused by HFD.
研究热量匹配的高脂饮食（HFD）对小鼠自发身体活动（SPA）、体重、炎症状态以及下丘脑能量稳态相关基因表达的影响。
将C57Bl/6小鼠（每组n = 5）分为两组，分别给予对照饮食（脂肪提供16.5%的热量）——对照组（C），或热量匹配的高脂饮食（脂肪提供60%的热量）。我们定期通过红外光束传感器评估体重和SPA，并在第12周结束时，通过酶联免疫吸附测定（ELISA）验证血糖水平、脂肪垫重量、血浆胰岛素、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6），并通过定量实时PCR阵列检测下丘脑84个与能量稳态相关基因的表达。
等热量的高脂饮食在最初48小时内就降低了SPA，并且与对照组相比，高脂饮食组的SPA在整个过程中一直较低。与对照组相比，这些变化导致高脂饮食组体重增加、肥胖、TNF-α和IL-6升高、血糖升高以及高胰岛素血症。高脂饮食喂养的小鼠下丘脑Agrp、脑源性神经营养因子（Bdnf）、肾上腺素能受体α2b（Adra2b）和酪酪肽（Pyy）基因的表达发生改变，突出了能量稳态关键调节因子Bdnf的下调。
膳食常量营养素分布在能量稳态中起着重要作用，这超出了其能量含量。尽管热量匹配，但高脂饮食由于自发身体活动减少导致体重增加和肥胖，突出了自发身体活动在能量平衡中的关键作用。下丘脑基因表达的变化似乎是高脂饮食导致自发身体活动减少的基础。
