Institute of Nutrition And Food Safety, Xiamen Medical College, Xiamen 361000, China; College of Food Science, South China Agricultural University, Guangzhou 510642, China.
College of Food and Biological Engineering, Jimei University, Xiamen 361000, China.
Food Chem. 2017 Sep 15;231:42-50. doi: 10.1016/j.foodchem.2017.03.121. Epub 2017 Mar 23.
Konjac glucomannan (KGM) is an important functional polysaccharide in food research. However, unstable dispersibility of KGM inhibits its in-depth study and wide application. In this study, a degraded KGM (100kGy-KGM), which showed excellent dispersibility and specific physicochemical properties, were obtained by γ-irradiation in a dosage of 100kGy. We investigated the protective effect of 100kGy-KGM against HO induced oxidative damage in LO2 cells. Our results demonstrated that pretreatment of LO2 cells with 100kGy-KGM not only significantly increased cellular survivals and activities of GSH-Px and CAT, but also reduced levels of LDH, MDA and intracellular accumulation of ROS. The marked protective effect against oxidative damage and excellent dispersibility in 100kGy-KGM allowed its possible use as an antioxidant. Our study provided fundamental knowledge to understand the structure-functions relationships of degraded-KGM, which could result in a theoretical guidance for the future application of KGM.
魔芋葡甘聚糖（KGM）是食品研究中一种重要的功能性多糖。然而，KGM 不稳定的分散性抑制了其深入研究和广泛应用。在这项研究中，通过剂量为 100kGy 的γ射线辐照，获得了具有优异分散性和特定物理化学性质的降解 KGM（100kGy-KGM）。我们研究了 100kGy-KGM 对 LO2 细胞中 HO 诱导的氧化损伤的保护作用。结果表明，用 100kGy-KGM 预处理 LO2 细胞不仅显著提高了细胞存活率和 GSH-Px 和 CAT 的活性，还降低了 LDH、MDA 的水平和 ROS 的细胞内积累。100kGy-KGM 对氧化损伤的显著保护作用和优异的分散性使其可能被用作抗氧化剂。我们的研究为了解降解-KGM 的结构-功能关系提供了基础知识，这可能为 KGM 的未来应用提供理论指导。
