接受血液透析的慢性肾脏病患者网织红细胞血红蛋白当量与网织红细胞血红蛋白含量的一致性

Concordance between Reticulocyte Hemoglobin Equivalent and Reticulocyte Hemoglobin Content in CKD Patients Undergoing Hemodialysis.

作者信息

Wirawan Riadi, Tedja Ailinda Theodora, Henrika Fify, Lydia Aida

机构信息

Department of Clinical Pathology, Faculty of Medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia.

出版信息

Acta Med Indones. 2017 Jan;49(1):34-40.

PMID:28450652

Abstract

AIM

to evaluate the correlation and the concordance between reticulocyte hemoglobin equivalent (RET-He) and reticulocyte hemoglobin content (CHr) as well as to obtain the cut-off value of RET-He as the target of iron supplementation in chronic kidney disease (CKD) patients undergoing hemodialysis.

METHODS

a cross-sectional study was performed using K3EDTA-anticoagulated peripheral blood samples collected from 106 CKD patients undergoing routine hemodialysis. The samples were then analyzed using both Sysmex XN-2000 and Siemens ADVIA 2120i for RET-He and CHr analysis.

RESULTS

a very strong correlation (r=0.91; p<0.0001) and a good concordance were found between RET-He and CHr with mean bias of 0.5 pg. The diagnostic concordance was 96.23%. The cut-off value of RET-He 29.2 pg was obtained from the receiver operating characteristic (ROC) curve with CHr as the gold standard. At this cut-off point, the sensitivity and specificity to assess the target of iron supplementation in CKD patients undergoing hemodialysis were 95.5% and 94%, respectively.

CONCLUSION

the study shows a good correlation and concordance between RET-He and CHr in CKD patients undergoing hemodialysis.

摘要

目的

评估网织红细胞血红蛋白当量（RET-He）与网织红细胞血红蛋白含量（CHr）之间的相关性和一致性，并得出RET-He作为接受血液透析的慢性肾脏病（CKD）患者铁补充目标的临界值。

方法

采用横断面研究，收集106例接受常规血液透析的CKD患者经K3EDTA抗凝的外周血样本。然后使用Sysmex XN-2000和西门子ADVIA 2120i对样本进行RET-He和CHr分析。

结果

RET-He与CHr之间存在非常强的相关性（r = 0.91；p < 0.0001）和良好的一致性，平均偏差为0.5 pg。诊断一致性为96.23%。以CHr为金标准，通过受试者工作特征（ROC）曲线得出RET-He的临界值为29.2 pg。在此临界值时，评估接受血液透析的CKD患者铁补充目标的敏感性和特异性分别为95.5%和94%。