Purification and crystallization of thymidine kinase from herpes simplex virus type 1.

作者信息

Sanderson M R, Freemont P S, Murthy H M, Krane J F, Summers W C, Steitz T A

机构信息

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CN 06511.

出版信息

J Mol Biol. 1988 Aug 20;202(4):917-9. doi: 10.1016/0022-2836(88)90569-4.

Thymidine kinase from herpes simplex virus type 1 (ATP:thymidine 5'-phosphotransferase; EC 2.7.1.21) has been purified from an overexpression system and crystallized against ammonium sulfate by using the hanging-drop technique. The tetragonal crystals are of space group P4122 or P4322, and have unit cell dimensions a = b = 84 A, c = 180 A.