Arkesteijn Irene T M, Potier Esther, Ito Keita
Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Department of Osteoarticular Bioengineering and Bioimaging, University Paris Diderot, Paris, France.
Global Spine J. 2017 Feb;7(1):14-20. doi: 10.1055/s-0036-1583174. Epub 2017 Feb 1.
In vitro disk explant culture.
Notochordal cells (NCs) have been shown to upregulate matrix production by nucleus pulposus (NP) cells in coculture. To examine the translation of these in vitro results to a nativelike setting, the regenerative potential of NCs injected into NP tissue was assessed in this study.
NP explants were cultured after injection with NCs in phosphate-buffered saline (PBS) or with PBS alone (sham). At days 0 and 42, cell viability and morphology, water, DNA, sulfated glycosaminoglycan and hydroxyproline content, and gene expression of anabolic markers were analyzed.
NCs remained viable during culture, but their morphology changed. The biochemical content remained unchanged, except for the DNA content in the NC group. Overall expression remained unchanged, whereas decreased during culture.
No overall anabolic response was observed when NCs were injected into NP explants. NCs were found to survive but did not display the typical NC morphology by the end of the culture period.
体外椎间盘外植体培养。
在共培养中，脊索细胞（NCs）已被证明可上调髓核（NP）细胞的基质产生。为了检验这些体外实验结果在类似天然环境中的转化情况，本研究评估了注入NP组织的NCs的再生潜力。
NP外植体在注射含NCs的磷酸盐缓冲盐水（PBS）或仅注射PBS（假手术组）后进行培养。在第0天和第42天，分析细胞活力和形态、水、DNA、硫酸化糖胺聚糖和羟脯氨酸含量以及合成代谢标志物的基因表达。
NCs在培养过程中保持存活，但其形态发生了变化。除NC组的DNA含量外，生化成分保持不变。总体表达保持不变，而在培养过程中下降。
将NCs注入NP外植体时未观察到总体合成代谢反应。发现NCs存活，但在培养期结束时未表现出典型的NC形态。
