Kamanna Sathisha, Henry Julianne, Voelcker Nicolas H, Linacre Adrian, Paul Kirkbride K
Flinders University, School of Chemical and Physical Sciences, GPO Box 2100, Adelaide, South Australia, 5001, Australia.
Forensic Science SA, Forensic Science Centre, Adelaide, South Australia, 5000, Australia.
Int J Legal Med. 2017 Sep;131(5):1413-1422. doi: 10.1007/s00414-017-1587-5. Epub 2017 Apr 27.
During a crime, biological material such as blood or vaginal fluid may become smeared on the fingers of the victim or suspect or trapped under their fingernails. The type of trapped fluid is extremely valuable forensic information. Furthermore, if either person touches an object at the crime scene with their 'contaminated' finger then a 'contaminated' finger mark may be deposited. Such marks have great value as they could identify not only who deposited the mark but also who they touched and which part of the body they touched. Here, we describe preliminary work towards a 'toolbox' of techniques based on mass spectrometry (MS) for the identification of biological fluid traces under fingernails or the imaging of them in finger marks. Liquid chromatography-multidimensional MS was effective for the detection of protein biomarkers characteristic of vaginal fluid and blood trapped under fingernails, even after hands had been washed. In regard to examination of finger marks for the presence of biological fluids, the most practical implementation of any technique is to integrate it with, but after, routine crime scene finger mark enhancement has been applied. Here, we demonstrate the usage of matrix-assisted laser desorption ionization-time of flight-MS for the detection and mapping of proteins and peptides from body fluids in finger marks, including marks enhanced using aluminium-containing magnetic powder and then 'lifted' with adhesive tape. Hitherto, only small molecules have been detected in enhanced, lifted marks. In a novel development, aluminium in the enhancement powder assisted ionization of small molecules in finger marks to the extent that conventional matrix was not required for MS.
在犯罪过程中，血液或阴道分泌物等生物材料可能会涂抹在受害者或嫌疑人的手指上，或被困在他们的指甲下。被困液体的类型是极其有价值的法医信息。此外，如果任何人用其“受污染”的手指触摸犯罪现场的物体，那么可能会留下“受污染”的指纹。这些指纹具有很大的价值，因为它们不仅可以识别留下指纹的人，还可以识别他们接触了谁以及接触了身体的哪个部位。在这里，我们描述了基于质谱（MS）的一系列技术“工具箱”的初步工作，用于识别指甲下的生物液体痕迹或对指纹中的这些痕迹进行成像。液相色谱 - 多维质谱对于检测被困在指甲下的阴道分泌物和血液特有的蛋白质生物标志物非常有效，即使手已经洗过。关于检查指纹中是否存在生物液体，任何技术最实际的应用是在应用常规犯罪现场指纹增强技术之后，将其与之结合。在这里，我们展示了基质辅助激光解吸电离飞行时间质谱用于检测和绘制指纹中来自体液的蛋白质和肽，包括使用含铝磁粉增强然后用胶带“提取”的指纹。迄今为止，在增强的、提取的指纹中仅检测到小分子。在一项新的进展中，增强粉末中的铝辅助指纹中小分子的电离，以至于质谱分析不需要传统的基质。
