Muthukumaran Chandrasekaran, Banupriya Loganathan, Harinee Somasundharam, Sivaranjani Sivagurunathan, Sharmila Govindasamy, Rajasekar Vinayagam, Kumar Narasimhan Manoj
Bioprocess Laboratory, Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamilnadu, 641 013, India.
Department of Genetic Engineering, SRM University, Kattankulathur, Tamilnadu, 603 203, India.
3 Biotech. 2017 May;7(1):66. doi: 10.1007/s13205-017-0655-3. Epub 2017 Apr 27.
Pectin derived from plant waste sources is currently focused as an economical and eco-friendly approach. Optimization of pectin extraction from muskmelon peel by response surface methodology (RSM) was investigated in this study. Box-Behnken Design (BBD) was used to identify the optimal level of the extraction variables such as time, pH and temperature. A second-order model equation for pectin extraction was obtained from multiple regression analysis of experimental data with the correlation coefficient (R ) value of 0.92. ANOVA results showed that linear effect of temperature and combined effect of pH with temperature were found significant for pectin extraction from muskmelon peel. Validation results had good agreement with the predicted results. Pectin extracted from muskmelon peel was classed as high methoxy pectin with the equivalent weight of 384.5 g/mol. Non-newtonian pseudoplastic flow behaviour was observed for muskmelon pectin from the viscosity studies.
目前，从植物废料中提取果胶是一种经济且环保的方法。本研究通过响应面法（RSM）对从甜瓜皮中提取果胶的工艺进行了优化。采用Box-Behnken设计（BBD）来确定提取变量（如时间、pH值和温度）的最佳水平。通过对实验数据进行多元回归分析，得到了果胶提取的二阶模型方程，相关系数（R）值为0.92。方差分析结果表明，温度的线性效应以及pH值与温度的联合效应对于从甜瓜皮中提取果胶具有显著影响。验证结果与预测结果吻合良好。从甜瓜皮中提取的果胶被归类为高甲氧基果胶，当量为384.5 g/mol。通过粘度研究发现，甜瓜果胶具有非牛顿假塑性流动行为。
