一种用于在酸性区域进行 pH 监测和成像的水溶性罗丹明 B 衍生荧光探针。

A water-soluble rhodamine B-derived fluorescent probe for pH monitoring and imaging in acidic regions.

机构信息

Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People's Republic of China.

出版信息

Methods Appl Fluoresc. 2017 Apr 28;5(2):024009. doi: 10.1088/2050-6120/aa69c7.

DOI:10.1088/2050-6120/aa69c7

PMID:28452333

Abstract

A structurally simple, water-soluble rhodamine-derivatived fluorescent probe, which is responsive to acidic pH, was conveniently synthesized via a one-step condensation reaction of rhodamine B hydrazide and 4-formybenzene-1,3-disulfonate. As a stable and highly sensitive pH sensor, the probe displays an approximately 50-fold fluorescence enhancement over the pH range of 7.16-4.89 as the structure of probe changes from spirocyclic (weak fluorescent) to ring-open (strong fluorescent) with decreasing pH. The synthesized fluorescent probe is applied to the detection of pH changes in vitro and in vivo bioimaging of immortalized gastric cancer cells, with satisfactory results.

摘要

一种结构简单、水溶性的罗丹明衍生荧光探针，可响应酸性 pH 值，通过一步缩合反应方便地合成，由罗丹明 B 腙和 4-甲酰基苯-1,3-二磺酸钠反应得到。作为一种稳定且高灵敏度的 pH 传感器，该探针在 pH 值范围为 7.16-4.89 时，其结构从螺环（弱荧光）变为开环（强荧光），荧光强度增强约 50 倍。合成的荧光探针可用于体外 pH 值变化的检测和永生胃癌细胞的体内生物成像，结果令人满意。