Sengupta Mandar, Raychaudhuri Sarmistha Sen
a Department of Biophysics, Molecular Biology and Bioinformatics , University of Calcutta , Kolkata , India.
Int J Radiat Biol. 2017 Aug;93(8):803-817. doi: 10.1080/09553002.2017.1321807. Epub 2017 May 16.
Environmental changes generate free radicals and reactive oxygen species (ROS) resulting in abiotic stress in plants. This causes alterations in germination, morphology, growth and development ultimately leading to yield loss. Gamma irradiation was used to experimentally induce oxidative damage in an important pulse crop Vigna radiata (L.) Wilczek or mung bean. Our research was aimed towards augmentation of oxidative stress tolerance through treatment with a group of aliphatic amines known as polyamines.
We used sub-lethal doses of gamma irradiation to generate oxidative damage which was evaluated using Nitro blue tetrazolium (NBT) staining, total antioxidant activity, 1, 1-Diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay, proline content and lipid peroxidation. Changes in internal free polyamines and messenger ribonucleic acid (mRNA) expression of key rate-limiting S-adenosylmethionine decarboxylase (SAMDC) enzyme in polyamine biosynthetic pathway was studied using real-time polymerase chain reaction (PCR).
We observed increased oxidative damage with higher irradiation dose which was partially alleviated by putrescine treatment. Internal levels of putrescine and spermidine increased with 1 mM (50 and 100 Gy) and 2 mM putrescine treatment. Expression of SAMDC also increased with putrescine treatment.
This study shows that treatment with putrescine can partially alleviate oxidative damage caused by gamma rays.
环境变化会产生自由基和活性氧（ROS），从而对植物造成非生物胁迫。这会导致种子萌发、形态、生长和发育发生改变，最终导致产量损失。本研究利用伽马射线辐照，对重要豆类作物绿豆（Vigna radiata (L.) Wilczek）进行实验性氧化损伤诱导。我们的研究旨在通过用一组称为多胺的脂肪族胺进行处理，增强其对氧化胁迫的耐受性。
我们使用亚致死剂量的伽马射线辐照来产生氧化损伤，并通过氮蓝四唑（NBT）染色、总抗氧化活性、1,1-二苯基-2-苦基肼（DPPH）自由基清除试验、脯氨酸含量和脂质过氧化作用来评估。利用实时聚合酶链反应（PCR）研究了多胺生物合成途径中关键限速酶S-腺苷甲硫氨酸脱羧酶（SAMDC）的内部游离多胺和信使核糖核酸（mRNA）表达的变化。
我们观察到，随着辐照剂量的增加，氧化损伤加剧，而腐胺处理可部分缓解这种损伤。经1 mM（50和100 Gy）和2 mM腐胺处理后，内部腐胺和亚精胺水平升高。腐胺处理后，SAMDC的表达也增加。
本研究表明，腐胺处理可部分缓解伽马射线造成的氧化损伤。
