Llados F T, Kriho V, Pappas G D
Department of Anatomy, University of Illinois, College of Medicine, Chicago.
Muscle Nerve. 1988 Sep;11(9):953-6. doi: 10.1002/mus.880110908.
Incubation of frog skeletal muscle with the ionophore A23187 induces severe damage in the muscle. At the level of the Z line, the ionophore induces redistribution and release of the protein alpha-actinin, as shown by immunocytochemical techniques. The ionophore does not induce damage in denervated preparations or in preparations pretreated with d-tubocurarine, which indicates that the effect is indirect and neurally mediated. It is concluded that the increase in spontaneous transmitter release produced by the ionophore induces Ca2+ influx through the acetylcholine receptor. Calcium ions activate neutral proteases which release alpha-actinin from the Z line.
用离子载体A23187孵育青蛙骨骼肌会导致肌肉严重损伤。在Z线水平，离子载体可诱导α - 辅肌动蛋白的重新分布和释放，免疫细胞化学技术已证实这一点。离子载体不会在去神经支配的制剂或用d - 筒箭毒碱预处理的制剂中诱导损伤，这表明该效应是间接的且由神经介导。得出的结论是，离子载体引起的自发递质释放增加会诱导Ca2+通过乙酰胆碱受体流入。钙离子激活中性蛋白酶，从而从Z线释放α - 辅肌动蛋白。
