Burgoyne R D, Pearce I A, Cambray-Deakin M
MRC Secretory Control Research Group, University of Liverpool, U.K.
Neurosci Lett. 1988 Aug 15;91(1):47-52. doi: 10.1016/0304-3940(88)90247-9.
The effects of glutamate receptor agonists on cytosolic calcium concentration in freshly isolated cerebellar granule cells and cells maintained in short term culture were determined using the fluorescent indicator fura-2. In freshly isolated cells depolarisation with high-K+ or addition of N-methyl-D-aspartate (NMDA) had no or only small effects on cytosolic calcium. After cells had been grown in monolayer culture for 20 h marked increases in cytosolic calcium concentration in response to 25 mm K+ or NMDA were detected. Other glutamate agonists had no (quisqualate) or small (kainate) effects on cytosolic calcium concentration. NMDA elevated cytosolic calcium concentration in the absence or presence of Mg2+ both before and after depolarisation by high K+. The results suggest that the effect of NMDA on granule cell differentiation and survival could be mediated by elevation of cytosolic calcium levels.
使用荧光指示剂fura-2测定了谷氨酸受体激动剂对新鲜分离的小脑颗粒细胞以及短期培养细胞胞质钙浓度的影响。在新鲜分离的细胞中，用高钾去极化或添加N-甲基-D-天冬氨酸（NMDA）对胞质钙没有影响或只有很小的影响。细胞在单层培养中生长20小时后，检测到对25 mM K+或NMDA反应时胞质钙浓度显著增加。其他谷氨酸激动剂对胞质钙浓度没有（quisqualate）或只有很小的（海人藻酸）影响。在高钾去极化之前和之后，无论有无Mg2+，NMDA均可提高胞质钙浓度。结果表明，NMDA对颗粒细胞分化和存活的影响可能是通过提高胞质钙水平来介导的。
