Yasui K, Masuda M, Matsuoka T, Yamazaki M, Komiyama A, Akabane T, Hasui M, Kobayashi Y, Murata K
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Pediatr Res. 1988 Oct;24(4):442-6. doi: 10.1203/00006450-198810000-00005.
Membrane properties associated with chemoattractant-mediated cellular responsiveness of neonatal polymorphonuclear leukocytes (PMN) were analyzed using n-formylmethionyl-leucyl-phenylalanine. Inasmuch as aliphatic alcohols as a membrane fluidizer can enhance the chemoattractant binding and affect subsequent cellular responsiveness in adult PMN, neonatal PMN were studied for such properties by their treatment with iso-propyl alcohol, an aliphatic alcohol. The alcohol (less than 2.5%) treatment enhanced the N-formylmethionyl-leucyl-phenylalanine binding to adult PMN, but there were no changes in the N-formylmethionyl-leucyl-phenylalanine binding to neonatal PMN. Although the N-formylmethionyl-leucyl-phenylalanine-induced subsequent responsiveness including migration, lysosomal enzyme release and superoxide anion production were modulated by the alcohol treatment in adult PMN, there was no such modulation in neonatal PMN. Because membrane fluidity is largely involved in the regulation of the receptor functions, the membrane fluidity of neonatal PMN was next measured by an excimer-forming lipid technique in flow cytometry. The membrane fluidity value (0.45 +/- 0.037) of neonatal PMN was lower than that (0.74 +/- 0.072) of adult PMN (p less than 0.01). Although the aliphatic alcohol enhanced the membrane fluidity of adult PMN, it did not affect the membrane fluidity of neonatal PMN. We conclude that there is abnormal membrane fluidity as a cause of impaired functional dynamics of the chemoattractant receptors, which appears to underlie the defective modulation of cell functions by the membrane fluidizer in neonatal PMN.
利用N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸分析了与新生儿多形核白细胞（PMN）趋化因子介导的细胞反应性相关的膜特性。由于脂肪醇作为膜流动性增强剂可增强趋化因子结合并影响成年PMN随后的细胞反应性，因此通过用脂肪醇异丙醇处理新生儿PMN来研究其此类特性。酒精（低于2.5%）处理增强了N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸与成年PMN的结合，但N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸与新生儿PMN的结合没有变化。尽管在成年PMN中，酒精处理可调节N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸诱导的包括迁移、溶酶体酶释放和超氧阴离子产生在内的后续反应性，但在新生儿PMN中没有这种调节作用。由于膜流动性在很大程度上参与受体功能的调节，接下来通过流式细胞术中的准分子形成脂质技术测量了新生儿PMN的膜流动性。新生儿PMN的膜流动性值（0.45±0.037）低于成年PMN的膜流动性值（0.74±0.072）（p<0.01）。尽管脂肪醇增强了成年PMN的膜流动性，但它不影响新生儿PMN的膜流动性。我们得出结论，膜流动性异常是趋化因子受体功能动力学受损的原因，这似乎是膜流动性增强剂对新生儿PMN细胞功能调节缺陷的基础。
