Yamamoto Ryota, Koyama Daisuke, Matsukawa Mami
Faculty of Life and Medical Sciences, Doshisha University, 1-3 Tataramiyakodani, Kyotanabe, Kyoto 610-0321, Japan; Wave Electronics Research Center, Doshisha University, 1-3 Tataramiyakodani, Kyotanabe, Kyoto 610-0321, Japan.
Wave Electronics Research Center, Doshisha University, 1-3 Tataramiyakodani, Kyotanabe, Kyoto 610-0321, Japan; Faculty of Science and Engineering, Doshisha University, 1-3 Tataramiyakodani, Kyotanabe, Kyoto 610-0321, Japan.
Ultrasonics. 2017 Aug;79:81-86. doi: 10.1016/j.ultras.2016.11.026. Epub 2016 Dec 15.
As biotechnology develops, techniques for manipulating and separating small particles such as cells and DNA are required in the life sciences. This paper investigates on-chip manipulation of microparticles in small channels by using ultrasonic vibration. The chip consists of a rectangular glass substrate with a cross-shaped channel (cross-section: 2.0×2.0mm) and four lead zirconate titanate transducers attached to the substrate's four corners. To efficiently generate the flexural vibration mode on the chip, we used finite element analysis to optimize the configurations of the glass substrate and transducers. Silicon carbide microparticles with an average diameter of 50μm were immersed in the channels, which were filled with ethanol. By applying an in-phase input voltage of 75V at 225kHz to the four transducers, a flexural vibration mode with a wavelength of 13mm was excited on the glass substrate, and this flexural vibration generated an acoustic standing wave in the channel. The particles could be trapped at the nodal lines of the standing wave. By controlling the driving phase difference between the two pairs of transducers, the vibrational distribution of the substrate could be moved along the channels so that the acoustic standing wave moved in the same direction. The trapped particles could be manipulated by the two-phase drive, and the transport direction could be switched at the junction of the channels orthogonally by changing the combination of the driving condition to four transducers.
随着生物技术的发展，生命科学领域需要能够操控和分离细胞及DNA等小颗粒的技术。本文研究了利用超声振动在小通道中对微粒进行芯片上的操控。该芯片由一个带有十字形通道（横截面：2.0×2.0毫米）的矩形玻璃基板以及附着在基板四个角上的四个锆钛酸铅换能器组成。为了在芯片上高效地产生弯曲振动模式，我们使用有限元分析来优化玻璃基板和换能器的配置。将平均直径为50μm的碳化硅微粒浸入充满乙醇的通道中。通过向四个换能器施加225kHz的75V同相输入电压，在玻璃基板上激发了波长为13mm的弯曲振动模式，并且这种弯曲振动在通道中产生了声驻波。微粒可以被困在驻波的波节线上。通过控制两对换能器之间的驱动相位差，基板的振动分布可以沿着通道移动，从而使声驻波向相同方向移动。被困的微粒可以通过两相驱动进行操控，并且通过改变对四个换能器的驱动条件组合，可以在通道的正交交汇处切换运输方向。
