He Jinpeng, Tian Ning, Yang Yanli, Jin Liangliang, Feng Xiu, Hua Junrui, Lin Sulan, Wang Bing, Li He, Wang Jufang
Gansu Key Laboratory of Space Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, P.R. China.
Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, P.R. China.
Oncol Lett. 2017 Apr;13(4):2442-2448. doi: 10.3892/ol.2017.5699. Epub 2017 Feb 8.
Melanoma is an aggressive malignancy that is increasingly common and exhibits a poor patient survival rate. Radiotherapy is the primary option for patients with melanoma, particularly those who are not candidates for surgery; however, the therapeutic effect is limited due to the relative radioresistance of melanoma to ionizing radiation (IR). It has been reported that microRNAs (miRNAs) serve a vital role in determining the radiosensitivity of tumors; however, little is known concerning the radiosensitization of melanoma using miRNA. In the present study, the radiosensitization effect of miRNA 185 (miR-185), which has been demonstrated to reduce renal cancer radioresistance, was investigated in B16 cells, a skin melanoma cell line derived from C57/BL mice, was investigated. Cell proliferation and scratch wound healing assays were used to determine the proliferative and migratory abilities of B16 cells. Annexin V/propidium iodide double staining was used to determine the apoptosis induced by IR. A tumor formation assay was performed to determine the radiosensitization effect of miR-185 on melanoma cells . Proliferation marker protein Ki-67, and hematoxylin and eosin staining were used to assess the proliferative activity and histological changes, respectively. The results of the present study demonstrated that miR-185 suppresses cellular proliferation and migration, and enhances IR-induced apoptosis, and the inhibition of proliferation and migration, and , which provides an insight into understanding the radiosensitization of melanoma using miRNA.
黑色素瘤是一种侵袭性恶性肿瘤，其发病率日益增加，患者生存率较低。放射治疗是黑色素瘤患者的主要治疗选择，尤其是那些不适合手术的患者；然而，由于黑色素瘤对电离辐射（IR）具有相对放射抗性，其治疗效果有限。据报道，微小RNA（miRNA）在决定肿瘤的放射敏感性方面起着至关重要的作用；然而，关于使用miRNA对黑色素瘤进行放射增敏的研究却很少。在本研究中，研究了已被证明可降低肾癌放射抗性的miRNA 185（miR-185）对B16细胞（一种源自C57/BL小鼠的皮肤黑色素瘤细胞系）的放射增敏作用。使用细胞增殖和划痕伤口愈合试验来确定B16细胞的增殖和迁移能力。采用膜联蛋白V/碘化丙啶双染法测定IR诱导的细胞凋亡。进行肿瘤形成试验以确定miR-185对黑色素瘤细胞的放射增敏作用。分别使用增殖标记蛋白Ki-67以及苏木精和伊红染色来评估增殖活性和组织学变化。本研究结果表明，miR-185可抑制细胞增殖和迁移，并增强IR诱导的细胞凋亡，以及对增殖和迁移的抑制作用，这为理解使用miRNA对黑色素瘤进行放射增敏提供了思路。
