Wang Ruizhong, Fang Hua, Fang Qin
Department of Laboratory, People's Hospital of Pudong New Area of Shanghai, Shanghai 201299, P.R. China.
Department of Hyperbaric Oxygen, People's Hospital of Pudong New Area of Shanghai, Shanghai 201299, P.R. China.
Oncol Lett. 2017 Apr;13(4):2811-2816. doi: 10.3892/ol.2017.5781. Epub 2017 Feb 28.
FK506 binding protein (FBBP) 14 belongs to the family of FKBPs. Altered expression of FKBPs are observed in several malignancies. The present study aimed to explore the expression and biological function of FKBP14 in gastric cancer. FKBP14 expression levels in 40 gastric cancer samples and matched control samples were evaluated using quantitative polymerase chain reaction. Cell proliferation was evaluated using Cell Counting kit-8 assay. A cell adhesion and a Transwell assay were performed to detect cell adhesion and invasion. Protein expression was determined using western blot analysis. It was found that FKBP14 expression in gastric cancer tissues was elevated compared with normal tissues. Silencing of FKBP14 expression in the gastric cancer MKN-45 and AGS cell lines, which have a higher expression level of FKBP14 compared with four other gastric cancer cell lines, significantly inhibited cellular proliferation, adhesion and invasion. In addition, the protein levels of proliferating cell nuclear antigen, matrix metalloproteinase 2 and the epithelial-mesenchymal-transition (EMT) markers β-catenin, Snail1 and Twist were repressed in gastric cancer cells with FKBP14 silenced. In conclusion, FKBP14 may act as an oncogene by suppressing cellular proliferation, adhesion and invasion and EMT in gastric carcinogenesis. FKBP14 may be a diagnosis marker and potential therapeutic target in gastric cancer.
FK506结合蛋白（FBBP）14属于FKBP家族。在多种恶性肿瘤中均观察到FKBP的表达发生改变。本研究旨在探讨FKBP14在胃癌中的表达及生物学功能。采用定量聚合酶链反应评估40例胃癌样本及配对对照样本中FKBP14的表达水平。使用细胞计数试剂盒-8法评估细胞增殖。进行细胞黏附实验和Transwell实验以检测细胞黏附和侵袭情况。采用蛋白质印迹分析确定蛋白表达。结果发现，与正常组织相比，胃癌组织中FKBP14的表达升高。在FKBP14表达水平高于其他四种胃癌细胞系的胃癌MKN-45和AGS细胞系中沉默FKBP14表达，可显著抑制细胞增殖、黏附和侵袭。此外，在FKBP14沉默的胃癌细胞中，增殖细胞核抗原、基质金属蛋白酶2以及上皮-间质转化（EMT）标志物β-连环蛋白、Snail1和Twist的蛋白水平均受到抑制。总之，FKBP14可能通过抑制胃癌发生过程中的细胞增殖、黏附、侵袭和EMT而发挥癌基因作用。FKBP14可能是胃癌的诊断标志物和潜在治疗靶点。
