Shen Jian Ying, Liao Jin Zhi, Guo Li Li, Su Rui Fang
Shanghai Jiaotong University, Department of Environmental Science and Resource, Shanghai, China.
Shanghai Jiaotong University, Department of Environmental Science and Resource, Shanghai, China.
Braz J Microbiol. 2017 Jul-Sep;48(3):544-550. doi: 10.1016/j.bjm.2016.10.028. Epub 2017 Mar 11.
Presence of the relatively new sulfonylurea herbicide monosulfuron-ester at 0.03-300nmol/L affected the growth of two non-target nitrogen-fixing cyanobacteria (Anabaena flos-aquae and Anabaena azotica) and substantially inhibited in vitro Acetolactate synthase activity, with IC of 3.3 and 101.3nmol/L for A. flos-aquae and A. azotica, respectively. Presenting in 30-300nmol/L, it inhibited protein synthesis of the cyanobacteria with less amino acids produced as its concentration increased. Our findings support the view that monosulfuron-ester toxicity in both nitrogen-fixing cyanobacteria is due to its interference with protein metabolism via inhibition of branch-chain amino acid biosynthesis, and particularly Acetolactate synthase activity.
相对较新的磺酰脲类除草剂单嘧磺酯以0.03 - 300nmol/L的浓度存在时，会影响两种非靶标固氮蓝藻（水华鱼腥藻和固氮鱼腥藻）的生长，并在体外显著抑制乙酰乳酸合酶的活性，对于水华鱼腥藻和固氮鱼腥藻，其半数抑制浓度（IC）分别为3.3和101.3nmol/L。当浓度为30 - 300nmol/L时，它会抑制蓝藻的蛋白质合成，且随着浓度增加，产生的氨基酸减少。我们的研究结果支持这样一种观点，即单嘧磺酯对固氮蓝藻的毒性是由于它通过抑制支链氨基酸生物合成，特别是乙酰乳酸合酶的活性来干扰蛋白质代谢。
