Sommella Eduardo, Pepe Giacomo, Pagano Francesco, Ostacolo Carmine, Tenore Gian Carlo, Russo Maria Teresa, Novellino Ettore, Manfra Michele, Campiglia Pietro
Department of Pharmacy, School of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, SA, Italy.
Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
Food Res Int. 2015 Oct;76(Pt 3):466-477. doi: 10.1016/j.foodres.2015.05.044. Epub 2015 May 29.
Annurca apple, a Southern Italian cultivar, possesses not only a particular taste and flavor, different from other types of apple, but also several healthy properties. With the aim to thoroughly elucidate the polyphenolic profile of this variety, listed as Protected Geographical Indication product, an extensive qualitative profiling of Annurca apple polyphenolic peel extract was carried out, by employing a combination of ultra high performance reversed phase (RP-UHPLC) and hydrophilic liquid chromatography (HILIC) coupled to ion trap-time of flight (IT-TOF) mass spectrometry. A total of 63 compounds were tentatively identified, 25 of which not reported in Annurca apple extract so far. Furthermore, thanks to the different selectivity obtained with the HILIC, in combination with accurate mass measurements, an improved separation and detection of procyanidins, was obtained. Moreover, the obtained profiles were compared with those of a conventional variety, such as Red Delicious (RD), highlighting their differences. This work contributes to increase the knowledge about the polyphenolic fingerprint of this typical apple variety.
阿努卡苹果是一种意大利南部的栽培品种，不仅具有与其他苹果品种不同的独特口感和风味，还具有多种健康特性。为了全面阐明这种被列为受保护地理标志产品的苹果品种的多酚谱，通过结合超高效反相（RP-UHPLC）和亲水液相色谱（HILIC）与离子阱飞行时间（IT-TOF）质谱联用，对阿努卡苹果多酚果皮提取物进行了广泛的定性分析。总共初步鉴定出63种化合物，其中25种迄今尚未在阿努卡苹果提取物中报道。此外，由于HILIC获得的不同选择性，结合精确质量测量，对原花青素实现了更好的分离和检测。此外，将所得图谱与传统品种如红富士（RD）的图谱进行比较，突出了它们之间的差异。这项工作有助于增加对这种典型苹果品种多酚指纹的了解。
