Perfilyeva Yuliya V, Abdolla Nurshat, Ostapchuk Yekaterina O, Tleulieva Raikhan, Krasnoshtanov Vladimir C, Belyaev Nikolai N
Laboratory of Molecular Immunology and Immunobiotechnology, M. A. Aitkhozhin's Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan.
Al-Farabi Kazakh National University, Almaty, Kazakhstan.
Inflamm Res. 2017 Aug;66(8):711-724. doi: 10.1007/s00011-017-1052-4. Epub 2017 Apr 28.
Myeloid-derived suppressor cells (MDSCs) are important negative regulators of immune processes in cancer and other pathological conditions. We suggested that MDSCs play a key role in pathogenesis of chronic inflammation, which precedes and, to a certain extent, induces carcinogenesis. The present study aimed at investigation of MDSCs arising during chronic inflammation and light-at-night (LN)-induced stress, which is shown to accelerate chronic diseases.
67 CD-1 mice and in vitro MDSC cultures.
Adjuvant arthritis was induced by a subdermal injection of complete Freund's adjuvant. LN was induced by illumination of 750 lx at night.
Flow cytometry for evaluation of cell phenotypes and MTT standard test for cell proliferation were used.
Increased levels of splenic CD11bLy6G and CD11bCD49d myeloid cells possessing suppressive potential in mice with adjuvant arthritis are shown. LN amplifies the process of CD11bLy6G expansion in mice with adjuvant arthritis. Expression of CD62L and CD195 is elevated on the myeloid cells during exposure to LN.
Our study raises the possibility that CD11bLy6G and CD11bCD49d MDSCs play an important role in the induction of immunosuppressive environment typical for chronic inflammation. Also, LN can affect immune responses during chronic inflammation through recruitment of MDSCs from the bone marrow.
髓系来源的抑制细胞（MDSCs）是癌症及其他病理状态下免疫过程的重要负调节因子。我们认为MDSCs在慢性炎症的发病机制中起关键作用，慢性炎症先于致癌作用并在一定程度上诱导致癌作用。本研究旨在调查慢性炎症及夜间光照（LN）诱导的应激过程中产生的MDSCs，夜间光照已被证明会加速慢性疾病的发展。
67只CD-1小鼠及体外MDSC培养物。
通过皮下注射完全弗氏佐剂诱导佐剂性关节炎。通过夜间750勒克斯的光照诱导夜间光照。
采用流式细胞术评估细胞表型，采用MTT标准试验检测细胞增殖。
结果显示，佐剂性关节炎小鼠脾脏中具有抑制潜能的CD11bLy6G和CD11bCD49d髓样细胞水平升高。夜间光照会增强佐剂性关节炎小鼠中CD11bLy6G的扩增过程。在暴露于夜间光照期间，髓样细胞上CD62L和CD195的表达升高。
我们的研究提出了一种可能性，即CD11bLy6G和CD11bCD49d MDSCs在诱导慢性炎症典型的免疫抑制环境中起重要作用。此外，夜间光照可通过从骨髓募集MDSCs来影响慢性炎症期间的免疫反应。
