Chun Sejong, Kim Eun-Young, Cha Seung-Yeon, Seo Ji-Young, Koo Hong Hoe, Cho Duck
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Transfus Apher Sci. 2017 Jun;56(3):427-430. doi: 10.1016/j.transci.2017.03.017. Epub 2017 Apr 7.
While a portable microscopic cell counter has been evaluated to enumerate residual white blood cells (WBCs) in red blood cells and platelet concentrates at blood centers, it has not yet been assessed in a hospital blood bank. We investigated the performance of this device and evaluated its accuracy, along with its benefits in time management. Residual WBCs from each of 100 apheresis platelet specimens were measured manually using a Nageotte chamber, along with flow cytometry methods and an ADAM-rWBC automated instrument (NanoEnTek, Seoul, South Korea). The efficiency was calculated by measuring the time required for the analysis of one specimen ten times consecutively. Flow cytometry and the ADAM-rWBC were able to detect four sporadic cases that had residual WBCs exceeding 1/μL that were not detected by the manual method. Analysis time was the shortest with the ADAM-rWBC, followed by flow cytometry and the manual method. Our data suggest that hospital blood banks require quality control of residual WBCs; among the methods evaluated in this study, the portable microscopic cell counter offers the best time efficiency.
虽然便携式显微镜细胞计数器已在血液中心用于计数红细胞和血小板浓缩物中的残留白细胞（WBC），但尚未在医院血库中进行评估。我们研究了该设备的性能，评估了其准确性以及在时间管理方面的优势。使用纳盖奥特计数板手动测量了100份单采血小板标本中的残留白细胞，同时采用了流式细胞术方法和ADAM-rWBC自动仪器（韩国首尔NanoEnTek公司）。通过连续十次测量分析一个标本所需的时间来计算效率。流式细胞术和ADAM-rWBC能够检测到4例残留白细胞超过1/μL的散发病例，而手动方法未检测到。ADAM-rWBC的分析时间最短，其次是流式细胞术和手动方法。我们的数据表明，医院血库需要对残留白细胞进行质量控制；在本研究评估的方法中，便携式显微镜细胞计数器具有最佳的时间效率。
