Hodgson Matthew J, Knutson Christopher C, Momtahan Nima, Cook Alonzo D
Brigham Young University, 350L CB, BYU, Provo, UT, 84602, USA.
Methods Mol Biol. 2018;1577:95-102. doi: 10.1007/7651_2017_31.
Decellularization of whole porcine hearts followed by recellularization with fully differentiated cells made from patient-specific human induced pluripotent stem cells (iPSCs) may provide the ultimate solution for patients with heart failure. Decellularization is the process of completely disrupting all cells and removing the cellular components (e.g., antigenic proteins, lipids, DNA) from organic tissue, leaving only the extracellular matrix (ECM). The decellularization of porcine hearts can be accomplished in 24 h and results in 98% DNA removal with only 6 h of detergent exposure. Automatically controlling the pressure during decellularization reduces the detergent exposure time while still completely removing immunogenic cell debris.
对整个猪心脏进行去细胞处理，然后用由患者特异性人类诱导多能干细胞（iPSC）制成的完全分化细胞进行再细胞化，这可能为心力衰竭患者提供最终解决方案。去细胞处理是指完全破坏所有细胞并从有机组织中去除细胞成分（如抗原性蛋白质、脂质、DNA），只留下细胞外基质（ECM）的过程。猪心脏的去细胞处理可在24小时内完成，仅需6小时的洗涤剂暴露即可实现98%的DNA去除。在去细胞处理过程中自动控制压力可减少洗涤剂暴露时间，同时仍能完全去除免疫原性细胞碎片。
