Uh Kyungjun, Lee Kiho
Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, USA.
Methods Mol Biol. 2017;1605:191-205. doi: 10.1007/978-1-4939-6988-3_13.
Maternal-to-zygotic transition is an event that developmental control of early embryos is switched from oocyte-derived factors to the zygotic genome. Ability to inhibit DNA replication, transcription, and translation is an important tool in studying events, such as zygotic genome activation, during embyogenesis. Here, we describe approaches to block DNA replication, transcription, and translation using chemical inhibitors. Then we also demonstrate how the transcript level of a maternally inherited gene, ten-eleven translocation methylcytosine dioxygenase 3, responses to the chemical treatments.
母源-合子转变是早期胚胎发育控制从卵母细胞衍生因子切换到合子基因组的一个事件。抑制DNA复制、转录和翻译的能力是研究胚胎发育过程中诸如合子基因组激活等事件的重要工具。在这里，我们描述了使用化学抑制剂阻断DNA复制、转录和翻译的方法。然后我们还展示了一个母源遗传基因——十一锌指甲基胞嘧啶双加氧酶3的转录水平如何对化学处理作出反应。
