Otomo Takanobu, Yoshimori Tamotsu
Department of Genetics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan.
Laboratory of Intracellular Membrane Dynamics, Osaka University Graduate School of Frontier Biosciences, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Methods Mol Biol. 2017;1594:141-149. doi: 10.1007/978-1-4939-6934-0_8.
Selective autophagy recognizes specific targets, including damaged mitochondria (mitophagy), aggregated proteins (aggrephagy), and invading bacteria (xenophagy) to engulf by isolation membrane, and degrades toxic materials within lysosomes. We recently revealed that a membrane-damaged lysosome itself also becomes a target of autophagy and named this process lysophagy. In this chapter, we describe methods for monitoring lysophagy; detecting lysosomal damage by staining of galectin and study the subsequent autophagic process in cultured mammalian cells.
选择性自噬识别特定靶标，包括受损的线粒体（线粒体自噬）、聚集的蛋白质（聚集体自噬）和入侵的细菌（异体自噬），通过隔离膜将其吞噬，并在溶酶体内降解有毒物质。我们最近发现，膜受损的溶酶体本身也会成为自噬的靶标，并将这一过程命名为溶酶体自噬。在本章中，我们描述了监测溶酶体自噬的方法；通过半乳糖凝集素染色检测溶酶体损伤，并研究培养的哺乳动物细胞中随后的自噬过程。
