Li Tao, Yang Guangming, Zhu Yu, Tzang Fei Chuen, Lau Sze-Hang, Kwok Sui-Yi, Wong Bing L, Liu Liangming
State Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, P.R. China.
New B Innovation Limited, Hong Kong, P.R. China.
J Surg Res. 2017 Apr;210:213-222. doi: 10.1016/j.jss.2016.11.045. Epub 2016 Nov 30.
To overcome the problems of previously reported hemoglobin-based oxygen carriers, we developed a stabilized nonpolymeric cross-linked tetrameric hemoglobin solution (YQ23). The aims of this study were to investigate the oxygen carrying and releasing properties of this novel hemoglobin-based oxygen carrier and to determine whether it has beneficial effects for hemorrhagic shock.
Using a hemorrhagic shock model in Sprague-Dawley rats and mini-pigs, we tested the effects of infusing 0.1, 0.3, and 0.5 g/kg YQ23 on animal survival, tissue oxygen delivery (DO) and consumption (VO), hemodynamics parameters, and liver, renal, and cardiac function.
YQ23 infusion increased the survival rate of rats and pigs with severe hemorrhagic shock in a dose-dependent manner. Moreover, it improved the hemodynamic parameters, cardiac output, DO and VO, and the mitochondrial respiratory function of vital organs. Among the three doses of YQ23, 0.5 gHb/kg YQ23 achieved a similar beneficial effect as whole blood.
This study indicated that the novel cross-linked tetrameric hemoglobin YQ23 has good oxygen carrying and releasing properties and exhibits beneficial effects on hemorrhagic shock in rats and pigs by improving the oxygen carrying and delivery function of blood, which maintains organ function.
为克服先前报道的基于血红蛋白的氧载体存在的问题，我们研发了一种稳定的非聚合交联四聚体血红蛋白溶液（YQ23）。本研究旨在探究这种新型基于血红蛋白的氧载体的携氧和释氧特性，并确定其对失血性休克是否具有有益作用。
利用Sprague-Dawley大鼠和小型猪的失血性休克模型，我们测试了输注0.1、0.3和0.5 g/kg YQ23对动物存活率、组织氧输送（DO）和消耗（VO）、血流动力学参数以及肝、肾和心脏功能的影响。
输注YQ23以剂量依赖方式提高了严重失血性休克大鼠和猪的存活率。此外，它改善了血流动力学参数、心输出量、DO和VO以及重要器官的线粒体呼吸功能。在YQ23的三个剂量中，0.5 gHb/kg YQ23产生了与全血相似的有益效果。
本研究表明，新型交联四聚体血红蛋白YQ23具有良好的携氧和释氧特性，通过改善血液的携氧和输送功能，对大鼠和猪的失血性休克表现出有益作用，从而维持器官功能。
