Shultz P J, DiCorleto P E, Silver B J, Abboud H E
Department of Medicine, Veterans Administration Medical Center, Cleveland, Ohio.
Am J Physiol. 1988 Oct;255(4 Pt 2):F674-84. doi: 10.1152/ajprenal.1988.255.4.F674.
Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and is released and/or synthesized by platelets, macrophages, endothelial cells, and rat mesangial cells. In the present investigation, we found that human glomerular mesangial cells in culture release a PDGF-like protein which competes for 125I-PDGF binding to human foreskin fibroblasts and is mitogenic for these fibroblasts. The competing and to a lesser extent the mitogenic activities present in the conditioned medium are partially recognized by an anti-PDGF antibody. Northern blot analysis of poly(A)+ RNA from human mesangial cells demonstrates the expression of both PDGF A- and B-chain mRNAs. PDGF also binds to mesangial cells in a specific manner and stimulates DNA synthesis and cell proliferation. These data suggest that a PDGF-like protein secreted by mesangial cells or released from platelets, monocytes, or endothelial cells during glomerular inflammation may function as an autocrine or a paracrine growth factor for these cells. The biological role of PDGF in mediating proliferative and other inflammatory events in the glomerulus remains to be identified.
血小板衍生生长因子（PDGF）是一种对间充质来源的细胞具有强大作用的促有丝分裂原，由血小板、巨噬细胞、内皮细胞和大鼠系膜细胞释放和/或合成。在本研究中，我们发现培养的人肾小球系膜细胞释放一种PDGF样蛋白，该蛋白可竞争125I-PDGF与人包皮成纤维细胞的结合，并对这些成纤维细胞具有促有丝分裂作用。条件培养基中存在的竞争活性以及程度较轻的促有丝分裂活性可被抗PDGF抗体部分识别。对人系膜细胞的聚腺苷酸加尾RNA（poly(A)+ RNA）进行Northern印迹分析，结果显示PDGF A链和B链mRNA均有表达。PDGF还以特异性方式与系膜细胞结合，并刺激DNA合成和细胞增殖。这些数据表明，系膜细胞分泌的或在肾小球炎症期间从血小板、单核细胞或内皮细胞释放的PDGF样蛋白可能作为这些细胞的自分泌或旁分泌生长因子发挥作用。PDGF在介导肾小球增殖和其他炎症事件中的生物学作用仍有待确定。
