Zhang Teng, Li Yuanyuan, Li Hui, Ma Xue-Shan, Ouyang Ying-Chun, Hou Yi, Schatten Heide, Sun Qing-Yuan
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
University of Chinese Academy of Sciences, Beijing 100101, China.
J Reprod Dev. 2017 Aug 19;63(4):383-388. doi: 10.1262/jrd.2017-018. Epub 2017 Apr 30.
LSM family member 14 (LSM14) belongs to the RNA-associated protein (RAP) family that is widely expressed in different species, and whose functions include associating and storing mRNAs. In the present study, we found that LSM14b was essential for oocyte meiotic maturation. Lack of LSM14b caused oocyte meiotic arrest at metaphase, and misalignment of chromosomes, as well as abnormal spindle assembly checkpoint (SAC) and maturation promoting factor (MPF) activation. Cyclin B1 and Cdc20 mRNAs, whose contents changed with LSM14b expression, were likely direct targets of LSM14b. We conclude that LSM14b, by functioning as a container of mRNAs, controls protein expression, and thus regulates the oocyte meiotic maturation process.
LSM家族成员14（LSM14）属于RNA相关蛋白（RAP）家族，该家族在不同物种中广泛表达，其功能包括结合和储存信使核糖核酸（mRNA）。在本研究中，我们发现LSM14b对卵母细胞减数分裂成熟至关重要。缺乏LSM14b会导致卵母细胞减数分裂停滞在中期，染色体排列紊乱，以及纺锤体组装检查点（SAC）和成熟促进因子（MPF）激活异常。细胞周期蛋白B1和Cdc20信使核糖核酸的含量随LSM14b表达而变化，它们可能是LSM14b的直接靶点。我们得出结论，LSM14b作为信使核糖核酸的载体，控制蛋白质表达，从而调节卵母细胞减数分裂成熟过程。
