Sorger S B, Matis L A, Engel I, McElligott D L, Fink P J, Hedrick S M
Department of Biology, University of California, San Diego, La Jolla 92093.
Ann N Y Acad Sci. 1988;532:18-32. doi: 10.1111/j.1749-6632.1988.tb36322.x.
We have previously described the B10.A pigeon cytochrome c-specific response in terms of clonal phenotypes and T-cell receptor (TcR) gene usage. All B10.A T-cell clones studied respond to antigen in association with syngeneic B10.A APCs and cross-react to antigen in association with one or two allogeneic variants of the I-E-encoded MHC molecules. In congenic strains of mice expressing these allogeneic MHC alleles [B10.A(5R) and B10.S(9R)], pigeon cytochrome c-specific T cells exhibit very similar MHC cross-reactivities. Our goal was to determine whether the same MHC cross-reactive T-cell clones were expressed in each appropriate strain, or whether each T-cell repertoire was unique. The results indicate that identical V alpha-J alpha and V beta-J beta combinations were expressed by the major pigeon cytochrome c-specific response phenotype in B10.A and B10.A(5R) mice. Previous functional data supports this overlap in expressed T-cell clones. B10.A and B10.S(9R) mice exhibit similar response phenotypes to pigeon cytochrome c but express distinctly different TcR genes. The results of these studies support the existence of at least two different mechanisms in determining MHC-linked immune response polymorphisms.
我们之前已根据克隆表型和T细胞受体（TcR）基因的使用情况描述了B10.A品系鸽子细胞色素c特异性应答。所有研究的B10.A T细胞克隆在与同基因B10.A抗原呈递细胞（APC）联合时对抗原产生应答，并在与I-E编码的MHC分子的一种或两种同种异体变体联合时与抗原发生交叉反应。在表达这些同种异体MHC等位基因的同基因小鼠品系[B10.A(5R)和B10.S(9R)]中，鸽子细胞色素c特异性T细胞表现出非常相似的MHC交叉反应性。我们的目标是确定相同的MHC交叉反应性T细胞克隆是否在每个合适的品系中表达，或者每个T细胞库是否是独特的。结果表明，B10.A和B10.A(5R)小鼠中主要的鸽子细胞色素c特异性应答表型表达相同的Vα-Jα和Vβ-Jβ组合。先前的功能数据支持了表达的T细胞克隆中的这种重叠。B10.A和B10.S(9R)小鼠对鸽子细胞色素c表现出相似的应答表型，但表达明显不同的TcR基因。这些研究结果支持在确定MHC连锁免疫应答多态性中至少存在两种不同机制。
