骨形态发生蛋白-7在 Göttingen 小型猪模型中维持可降解陶瓷颅骨成形术的表面完整性
BMP-7 Preserves Surface Integrity of Degradable-ceramic Cranioplasty in a Göttingen Minipig Model.
作者信息
Roldán J Camilo, Schulz Peter, Klünter Tim, Deisinger Ulrike, Diez Claudius, Waiss Waltraud, Kirschneck Christian, Reichert Torsten E, Detsch Rainer
机构信息
Division of Pediatric Facial Plastic Surgery and Craniofacial Anomalies, Catholic Children's Hospital Wilhelmstift, Hamburg, Germany; Teaching Hospital of the University of Lübeck, Lübeck, Germany; Department of Cranio-Maxillofacial Plastic Surgery, University of Regensburg, Regensburg, Germany; Friedrich-Baur-Research-Institute for Biomaterials, Bayreuth, Germany; Department of Cardiothoracic Surgery, University of Regensburg, Regensburg, Germany; Department of Orthodontics, University of Regensburg, Regensburg, Germany; and Department of Biomaterials, Cell Biology and Tissue Engineering, University of Erlangen, Erlangen, Germany.
出版信息
Plast Reconstr Surg Glob Open. 2017 Mar 16;5(3):e1255. doi: 10.1097/GOX.0000000000001255. eCollection 2017 Mar.
BACKGROUND
The aim of the study was to evaluate the integrity of a craniotomy grafted site in a minipig model using different highly porous calcium phosphate ceramic scaffolds either loaded or nonloaded with bone morphogenetic protein-7 (BMP-7).
METHODS
Four craniotomies with a diameter of 15 mm (critical-size defect) were grafted with different highly porous (92-94 vol%) calcium phosphate ceramics [hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP; a mixture of HA and TCP)] in 10 Göttingen minipigs: (a) group I (n = 5): HA versus BCP; (b) group II (n = 5): TCP versus BCP. One scaffold of each composition was supplied with 250 μg of BMP-7. In vivo computed tomography scan and fluorochrome bone labeling were performed. Specimens were evaluated 14 weeks after surgery by environmental scanning electron microscopy, fluorescence microscopy, and Giemsa staining histology.
RESULTS
BMP-7 significantly enhanced bone formation in TCP ( = 0.047). Slightly enhanced bone formation was observed in BCP ( = 0.059) but not in HA implants. BMP-7 enhanced ceramic degradation in TCP ( = 0.05) and BCP ( = 0.05) implants but not in HA implants. Surface integrity of grafted site was observed in all BMP-7-loaded implants after successful creeping substitution by the newly formed bone. In 9 of 10 HA implants without BMP-7, partial collapse of the implant site was observed. All TCP implants without BMP-7 collapsed. Fluorescent labeling showed bone formation at week 1 in BMP-7-stimulated implants.
CONCLUSIONS
BMP-7 supports bone formation, ceramic degradation, implant integration, and surface integrity of the grafted site.
背景
本研究的目的是在小型猪模型中，使用不同的高孔隙率磷酸钙陶瓷支架（负载或未负载骨形态发生蛋白-7（BMP-7））评估开颅移植部位的完整性。
方法
在10只哥廷根小型猪中，对4个直径为15毫米的开颅术（临界尺寸缺损）移植不同的高孔隙率（92 - 94体积%）磷酸钙陶瓷[羟基磷灰石（HA）、磷酸三钙（TCP）和双相磷酸钙（BCP；HA和TCP的混合物）]：（a）第一组（n = 5）：HA与BCP对比；（b）第二组（n = 5）：TCP与BCP对比。每种成分的一个支架负载250μg的BMP-7。进行体内计算机断层扫描和荧光染料骨标记。术后14周通过环境扫描电子显微镜、荧光显微镜和吉姆萨染色组织学对标本进行评估。
结果
BMP-7显著增强了TCP中的骨形成（P = 0.047）。在BCP中观察到骨形成略有增强（P = 0.059），但在HA植入物中未观察到。BMP-7增强了TCP（P = 0.05）和BCP（P = 0.05）植入物中的陶瓷降解，但在HA植入物中未增强。在新形成的骨成功进行爬行替代后，在所有负载BMP-7的植入物中观察到移植部位的表面完整性。在10个未负载BMP-7的HA植入物中的9个中，观察到植入部位部分塌陷。所有未负载BMP-7的TCP植入物均塌陷。荧光标记显示在BMP-7刺激的植入物中第1周有骨形成。
结论
BMP-7支持骨形成、陶瓷降解、植入物整合以及移植部位的表面完整性。
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