Frenkiel Brett A, Temple-Smith Peter, de Kretser David, Southwick Graeme J
Department of Obstetrics and Gynaecology, School of Clinical Studies at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, Australia; Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, Australia; The Hudson Institute, Melbourne, Australia; and Melbourne Institute of Plastic Surgery, Melbourne, Australia.
Plast Reconstr Surg Glob Open. 2017 Mar 1;5(3):e1258. doi: 10.1097/GOX.0000000000001258. eCollection 2017 Mar.
Breast capsular contracture remains an elusive problem faced by plastic surgeons and is the leading long-term complication after breast implantation. Follistatin (Fst) is a protein with known anti-inflammatory and antifibrotic properties and has the potential to limit the severity of diseases associated with inflammation and fibrosis such as capsular contracture. The aim of this study was to examine the effect of Fst288 on capsular fibrosis around silicone implants in a mouse model.
BALB/c mice were implanted subcutaneously with untreated silicone implants (baseline control). In the experimental group, immediately after silicone implant insertion, the implant pocket received either a single injection of 1 µg Fst288 or normal saline (internal control). The animals were killed at 3, 5, 7, 14, 28, and 90 days after surgery, and serum, implants, and the surrounding tissue were removed for histological and immunohistochemical analyses.
Fst288 treatment resulted in significant decreases in capsule thickness at 28 days ( < 0.05) and 3 months ( < 0.001), decreased collagen production at 14 days ( < 0.05) and 3 months ( < 0.01), decreased angiogenesis at 3 months ( < 0.001), decreased α-smooth muscle actin levels at 3 months ( < 0.05), and a decrease in the number of CD45+ cells at days 5 ( < 0.05) and 7 ( < 0.01), respectively, when compared with control implants.
A single injection of Fst288 at the time of silicone implant insertion into the mice results in a significant reduction in pericapsular inflammation and capsular fibrosis.
乳房包膜挛缩仍然是整形外科医生面临的一个难以捉摸的问题，并且是乳房植入术后主要的长期并发症。卵泡抑素（Fst）是一种具有已知抗炎和抗纤维化特性的蛋白质，有潜力限制与炎症和纤维化相关疾病（如包膜挛缩）的严重程度。本研究的目的是在小鼠模型中检测Fst288对硅胶植入物周围包膜纤维化的影响。
将未处理的硅胶植入物皮下植入BALB/c小鼠（基线对照）。在实验组中，硅胶植入物插入后立即在植入腔隙单次注射1μg Fst288或生理盐水（内部对照）。在手术后3、5、7、14、28和90天处死动物，并取出血清、植入物和周围组织进行组织学和免疫组化分析。
与对照植入物相比，Fst288治疗导致28天时包膜厚度显著降低（<0.05），3个月时显著降低（<0.001）；14天时胶原蛋白生成减少（<0.05），3个月时减少（<0.01）；3个月时血管生成减少（<0.001）；3个月时α-平滑肌肌动蛋白水平降低（<0.05）；5天时（<0.05）和7天时（<0.01）CD45+细胞数量分别减少。
在小鼠硅胶植入时单次注射Fst288可显著减轻包膜周围炎症和包膜纤维化。
