Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of MedicineSeoul, South Korea.
Institute for Medical Device Clinical Trials, Korea University College of MedicineSeoul, South Korea.
Front Cell Infect Microbiol. 2017 Apr 18;7:125. doi: 10.3389/fcimb.2017.00125. eCollection 2017.
(SA) and (PA) are known to cause biofilm-related infections. MRSA and PA have been frequently isolated from chronically infected wounds, cystic fibrosis, chronic suppurative otitis media (CSOM), and from indwelling medical devices, and these bacteria co-exist; however, their interaction with each-other or with the host is not well known. In this study, we investigated MRSA and PA multi-species biofilm communities and their interaction with the host during colonization using an OM rat-model. biofilm formation and colonization were studied using CV-microtiter plate assay and OM rat-model respectively. The biofilms were viewed under scanning electron microscope and bacteria were enumerated using cfu counts. The differential gene expressions of rat mucosa colonized with single or multi-species of MRSA or PA were studied using RNA-sequencing of total transcriptome. In multi-species biofilms PA partially inhibited SA growth. However, no significant inhibition of MRSA was detected during colonization of multi-species in rat bullae. A total of 1,797 genes were significantly ( < 0.05) differentially expressed in MRSA or PA or MRSA + PA colonized rat middle ear mucosa with respect to the control. The poly-microbial colonization of MRSA and PA induced the differential expression of a significant number of genes that are involved in immune response, inflammation, signaling, development, and defense; these were not expressed with single species colonization by either MRSA or PA. Genes involved in defense, immune response, inflammatory response, and developmental process were exclusively up-regulated, and genes that are involved in nervous system signaling, development and transmission, regulation of cell growth and development, anatomical and system development, and cell differentiation were down-regulated after multi-species inoculation. These results indicate that poly-microbial colonization induces a host response that is different from that induced by single species infection.
（SA）和（PA）已知会引起生物膜相关感染。耐甲氧西林金黄色葡萄球菌（MRSA）和 PA 经常从慢性感染的伤口、囊性纤维化、慢性化脓性中耳炎（CSOM）和留置的医疗设备中分离出来，这些细菌共存；然而，它们之间的相互作用或与宿主的相互作用尚不清楚。在这项研究中，我们使用 OM 大鼠模型研究了 MRSA 和 PA 多物种生物膜群落及其在定植过程中与宿主的相互作用。使用 CV 微量滴定板测定法和 OM 大鼠模型分别研究了生物膜形成和定植。使用扫描电子显微镜观察生物膜，并使用 CFU 计数对细菌进行计数。使用总转录组的 RNA 测序研究了单一或多种 MRSA 或 PA 定植大鼠黏膜的差异基因表达。在多物种生物膜中，PA 部分抑制了 SA 的生长。然而，在大鼠中耳泡中多物种定植期间，MRSA 的生长没有明显抑制。与对照相比，在 MRSA 或 PA 或 MRSA+PA 定植的大鼠中耳黏膜中，共有 1797 个基因的表达有显著差异（<0.05）。MRSA 和 PA 的多微生物定植诱导了大量参与免疫反应、炎症、信号转导、发育和防御的基因的差异表达，这些基因在单一物种定植时均未表达。与单一物种定植相比，涉及防御、免疫反应、炎症反应和发育过程的基因被特异性上调，而涉及神经系统信号转导、发育和传递、细胞生长和发育调节、解剖和系统发育以及细胞分化的基因被下调。这些结果表明，多微生物定植诱导的宿主反应与单一物种感染诱导的反应不同。
