Jun Yonghyun, Youn Cha Kyung, Jo Eu-Ri, Cho Sung Il
Department of Anatomy, Chosun University College of Medicine, Gwangju, South Korea.
Department of Premedical Science, Chosun University College of Medicine, Gwangju, South Korea.
Int J Pediatr Otorhinolaryngol. 2019 Nov;126:109622. doi: 10.1016/j.ijporl.2019.109622. Epub 2019 Aug 6.
Biofilm formation in tympanostomy tubes causes persistent and refractory otorrhea. In the present study, we investigated the in vitro antibiofilm activity of N-acetylcysteine (NAC) against biofilm formation by methicillin-resistant Staphylococcus aureus (MRSA) and quinolone-resistant Pseudomonas aeruginosa (QRPA).
We examined the antibiofilm activity of NAC against biofilms produced by MRSA and QRPA strains using in vitro biofilm formation assay, adhesion assay, and biofilm eradication assay. Additionally, the antibiofilm activity of different concentrations of NAC against tympanostomy-tube biofilms from MRSA and QRPA strains was compared using a scanning electron microscope.
The adhesion of MRSA and QRPA strains decreased significantly in a concentration-dependent manner after treatment with varying amounts of NAC. Treatment with NAC inhibited biofilm formation of both MRSA and QRPA strains and increased eradication of preformed mature biofilm produced by MRSA and QRPA. Besides, NAC exhibited significant eradication-activity against tympanostomy-tube biofilms produced by MRSA and QRPA strains.
Our results show potent inhibition of MRSA and QRPA biofilm after treatment with NAC. NAC shows potential for the treatment of biofilms and refractory post-tympanostomy tube otorrhea resulting from MRSA and QRPA infection.
鼓膜置管中的生物膜形成会导致持续性难治性耳漏。在本研究中，我们调查了N-乙酰半胱氨酸（NAC）对耐甲氧西林金黄色葡萄球菌（MRSA）和耐喹诺酮铜绿假单胞菌（QRPA）生物膜形成的体外抗生物膜活性。
我们使用体外生物膜形成试验、黏附试验和生物膜清除试验，检测了NAC对MRSA和QRPA菌株产生的生物膜的抗生物膜活性。此外，使用扫描电子显微镜比较了不同浓度的NAC对MRSA和QRPA菌株鼓膜置管生物膜的抗生物膜活性。
用不同量的NAC处理后，MRSA和QRPA菌株的黏附以浓度依赖性方式显著降低。NAC处理抑制了MRSA和QRPA菌株的生物膜形成，并增加了对MRSA和QRPA产生的预先形成的成熟生物膜的清除。此外，NAC对MRSA和QRPA菌株产生的鼓膜置管生物膜表现出显著的清除活性。
我们的结果表明，用NAC处理后，MRSA和QRPA生物膜受到有效抑制。NAC显示出治疗由MRSA和QRPA感染引起的生物膜和难治性鼓膜置管后耳漏的潜力。
