Kim Kang-San, Jung Jae-Ho, Min Gi-Sik
Department of Biological Sciences, Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, South Korea.
Department of Biology, Gangneung-Wonju National University, Jukheon-gil, 6, Gangneung-si, Gangwon-do, 25457, South Korea.
J Eukaryot Microbiol. 2017 Nov;64(6):873-884. doi: 10.1111/jeu.12421. Epub 2017 May 25.
Two new urostylid species, Holostichides heterotypicus n. sp. and Holosticha muuiensis n. sp., were discovered in South Korea. Morphological and phylogenetic analyses were carried out to confirm that these species are new to science. Holostichides heterotypicus is mainly characterized by the following combination of features: 110-205 μm long in vivo; 5-10 frontoterminal cirri; 6-8 midventral pairs with 2-3 midventral cirral rows; cortical granules present; four bipolar dorsal kineties; and 6-9 caudal cirri. Ontogenetic features of H. heterotypicus are similar to those of H. typicus. Phylogenetic analyses revealed that H. heterotypicus was distantly separated from bakuellid genera Apobakuella, Bakuella, Metaurostylopsis, and Neobakuella. This result is supported by the following features: transverse cirri (present in the other four bakuellids vs. absent in Holostichides) and caudal cirri (absent in the other four bakuellids vs. present in Holostichides). Holosticha muuiensis n. sp. is mainly distinguished from its congeners by the following combination of features: 100-185 long in vivo; shortened undulating membrane; cortical granules lacking; contractile vacuole absent; 51-66 adoral zone of membranelles; 42-60 macronuclear nodules; and five bipolar dorsal kineties. In the phylogenetic tree, Holosticha muuiensis n. sp. clustered with a Holosticha group (containing Holosticha diademata, Holosticha foissneri, and Holosticha heterofoissneri).
在韩国发现了两种新的尾柱虫物种，异型全列虫（Holostichides heterotypicus）新种和牟伊尾柱虫（Holosticha muuiensis）新种。进行了形态学和系统发育分析以确认这些物种是科学上新发现的。异型全列虫的主要特征如下：活体时长110 - 205微米；额前触毛5 - 10根；腹中部有6 - 8对，具2 - 3列腹中部触毛；有皮层颗粒；有4列双极背纤毛；尾触毛6 - 9根。异型全列虫的个体发育特征与典型全列虫相似。系统发育分析表明，异型全列虫与巴库虫属的阿波巴库虫、巴库虫、后尾柱虫属和新巴库虫属亲缘关系较远。这一结果得到以下特征的支持：横触毛（在其他四种巴库虫中有而在全列虫中没有）和尾触毛（在其他四种巴库虫中没有而在全列虫中有）。牟伊尾柱虫新种主要与其同属物种的区别在于以下特征组合：活体时长100 - 185；波动膜缩短；无皮层颗粒；无收缩泡；口围带膜粒51 - 66片；大核结节42 - 60个；有5列双极背纤毛。在系统发育树中，牟伊尾柱虫新种与一个尾柱虫类群（包含冠突尾柱虫、福氏尾柱虫和异福氏尾柱虫）聚类在一起。
