用于评估马钱子诱导器官毒性的斑马鱼模型。
Zebrafish model for assessing induced organ toxicity by Strychnos nux-vomica.
作者信息
Zhao Chongjun, Tian Jinghuan, Wang Jinfeng, Feng Yaru, Ni Yuanyuan, Fan Jiaojiao, Wang Chunmei, Cao Dan, Zou Qinwen, Ma Zhiqiang, Lin Ruichao
出版信息
J Tradit Chin Med. 2016 Aug;36(4):522-9. doi: 10.1016/s0254-6272(16)30070-x.
OBJECTIVE
To assess the acute organ toxicity of Strychnos nux-vomica with zebrafish model visually.
METHODS
To assess acute toxicity, we initially determined the lethal concentration after Strychnos nux-vomica treatment for 24 h. Zebrafish was treated with five concentrations ≦ LC10 for 24 h, and the effects of Strychnos nux-vomica on morphology, function of heart, central nervous system, liver, kidney and organ-specific cell death were assessed. Next, we assessed the reversibility of toxic effect.
RESULTS
Strychnos nux-vomica has an effect on the different organs of zebrafish, including heart, central nervous system, liver, and kidney, and cadiotoxicity induced by Strychnos nux-vomica was reversible to some extent.
CONCLUSION
Zebrafish model is suitable for confirming the toxic target organs for Chinese traditional medicine.
目的
采用斑马鱼模型直观评估马钱子的急性器官毒性。
方法
为评估急性毒性，我们首先测定马钱子处理24小时后的致死浓度。用五个浓度≤LC10的马钱子处理斑马鱼24小时，评估马钱子对形态、心脏功能、中枢神经系统、肝脏、肾脏及器官特异性细胞死亡的影响。接下来，我们评估毒性作用的可逆性。
结果
马钱子对斑马鱼的不同器官有影响，包括心脏、中枢神经系统、肝脏和肾脏，且马钱子诱导的心脏毒性在一定程度上是可逆的。
结论
斑马鱼模型适用于确定中药的毒性靶器官。
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