Tomisawa H, Takanohashi Y, Ichihara S, Fukazawa H, Tateishi M
Department of Drug Metabolism, Nippon Roche Research Center, Kanagawa Pref., Japan.
Biochem Biophys Res Commun. 1988 Sep 30;155(3):1119-25. doi: 10.1016/s0006-291x(88)81256-7.
Leukotriene E4 (LTE4) was transaminated to 5(S)-hydroxy-6(R)-S-(2-keto-3-thiopropionyl)-7,9-trans-11,14-cis- eicosatetraenoic acid (tentatively designated as LTG4) by cysteine conjugate aminotransferase I purified from rat liver supernatant in the presence of alpha-ketoglutaric acid or alpha-keto-gamma-methiolbutyric acid. The transamination activity was present in the kidney as well as in the liver, but not in the lung or leukocytes.
在存在α-酮戊二酸或α-酮-γ-甲硫基丁酸的情况下，从大鼠肝脏上清液中纯化的半胱氨酸结合转氨酶I将白三烯E4（LTE4）转氨生成5(S)-羟基-6(R)-S-(2-酮-3-硫代丙酰基)-7,9-反式-11,14-顺式-二十碳四烯酸（暂定为LTG4）。转氨活性存在于肾脏和肝脏中，但不存在于肺或白细胞中。
