PC12细胞中的心钠素高亲和力受体。

High affinity receptors for atrial natriuretic factor in PC12 cells.

作者信息

Rathinavelu A, Isom G E

机构信息

Department of Pharmacology and Toxicology, School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, IN 47907.

出版信息

Biochem Biophys Res Commun. 1988 Oct 14;156(1):78-85. doi: 10.1016/s0006-291x(88)80807-6.

DOI:10.1016/s0006-291x(88)80807-6

PMID:2845987

Abstract

Specific receptors for atrial natriuretic factor (ANF) are characterized on PC12 cells (rat pheochromocytoma cell line). Radioiodinated synthetic ANF (Rat, 8-33) bound to a single class of high affinity binding sites with the Kd value of 6.7 X 10(-10) M. The Bmax value was 29 fmol/10(5) cells and receptor density was calculated as 194,000 +/- 20,000/cell. Photoaffinity labelling of ANF receptor specifically labelled two protein bands with apparent m.wt of 70,000 and 130,000. When the cells were incubated with the labelled ligand at 37 degrees C the ligand was internalized. The rate of internalization increased in the presence of increased ligand concentration. ANF receptors on PC12 cells are reported for the first time which would provide a unique model for study of ANF-receptor interaction.

摘要

在PC12细胞（大鼠嗜铬细胞瘤细胞系）上对心房利钠因子（ANF）的特异性受体进行了表征。放射性碘化的合成ANF（大鼠，8 - 33）与一类高亲和力结合位点结合，解离常数（Kd）值为6.7×10⁻¹⁰ M。最大结合容量（Bmax）值为29 fmol/10⁵个细胞，受体密度计算为194,000 ± 20,000/细胞。ANF受体的光亲和标记特异性地标记了两条表观分子量为70,000和130,000的蛋白条带。当细胞在37℃与标记配体孵育时，配体被内化。内化速率在配体浓度增加时升高。首次报道了PC12细胞上的ANF受体，这将为研究ANF - 受体相互作用提供一个独特的模型。