Paolino Donatella, Accolla Maria Lorena, Cilurzo Felisa, Cristiano Maria Chiara, Cosco Donato, Castelli Francesco, Sarpietro Maria Grazia, Fresta Massimo, Celia Christian
Department of Experimental and Clinical Medicine, University of Catanzaro "Magna Graecia", Building of BioSciences, V.le Europa, I - 88100 Germaneto, Catanzaro, CZ, Italy; IRC-FSH-Interregional Research Center for Food Safety & Health, University of Catanzaro "Magna Græcia", Building of BioSciences, V.le Europa, I- 88100 Germaneto, Catanzaro, CZ, Italy.
Department of Health Sciences, University of Catanzaro "Magna Graecia", Building of BioSciences, V.le Europa, I - 88100 Germaneto, Catanzaro, CZ, Italy; Department of Drug Sciences, University of Catania, V.le A. Doria 6, I - 95125 Catania, Italy.
Colloids Surf B Biointerfaces. 2017 Jul 1;155:266-275. doi: 10.1016/j.colsurfb.2017.04.018. Epub 2017 Apr 13.
The degree to which liposomes are PEGylated is the feature, which most influences the length of the presence of stealth liposomes in the bloodstream. In order to thoroughly investigate the maximum amount of DSPE-PEG that can be used to stabilize stealth liposomes, these were synthesized at different concentrations of DSPE-PEG and their physicochemical properties were investigated by using differential scanning calorimetry (DSC). The kinetics of PEGylation and de-PEGylation were performed by incubating non-stealth liposomes in a DSPE-PEG suspension at different incubation times, and then analyzing the data using DSC and dynamic light scattering (DLS) techniques. The results demonstrated that DSPE-PEG was self-assembled in the phospholipid bilayers, thus forming stealth liposomes. The different amounts of DSPE-PEG in the bilayer triggered a de-PEGylation phenomenon, resulting in mixed nanoaggregates, which derived from the detergent-like properties of the PEGylated phospholipids.
脂质体聚乙二醇化的程度是最能影响隐形脂质体在血流中存在时间长短的特征。为了全面研究可用于稳定隐形脂质体的DSPE-PEG的最大用量，在不同DSPE-PEG浓度下合成了隐形脂质体，并通过差示扫描量热法（DSC）研究了它们的物理化学性质。通过将非隐形脂质体在DSPE-PEG悬浮液中于不同孵育时间孵育，然后使用DSC和动态光散射（DLS）技术分析数据，进行了聚乙二醇化和去聚乙二醇化的动力学研究。结果表明，DSPE-PEG在磷脂双层中自组装，从而形成隐形脂质体。双层中不同量的DSPE-PEG引发了去聚乙二醇化现象，导致形成混合纳米聚集体，这源于聚乙二醇化磷脂的去污剂样性质。
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