Zhu Jiqiao, Zeng Ye, Dolff Sebastian, Bienholz Anja, Lindemann Monika, Brinkhoff Alexandra, Schedlowski Manfred, Xu Shilei, Sun Ming, Guberina Hana, Kirchhof Julia, Kribben Andreas, Witzke Oliver, Wilde Benjamin
Department of Nephrology, University Hospital Essen, University Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany.
Department of Nephrology, University Hospital Essen, University Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany; Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, #100 Xianggang Road, Jiang'an District, Wuhan, Hubei, China.
Clin Immunol. 2017 Nov;184:48-53. doi: 10.1016/j.clim.2017.04.016. Epub 2017 Apr 28.
A separate subset of Granzyme B (GrB) producing B-cells regulating T-cell mediated immunity has been identified. In the present study, we investigated the role of GrB B-cells in renal transplant patients (RTX).
12 healthy controls (HC) and 26 RTX patients were enrolled. In addition, 19 healthy volunteers treated with cyclosporine A (CsA) were enrolled. GrB B-cells were determined via flow cytometry.
RTX Patients showed a diminished fraction of GrB B-cells as compared to HC. CsA treatment of healthy volunteers had no impact on the development of GrB B-cells. RTX patients with a history of allograft rejection showed an increased frequency of GrB B-cells. RTX patients with at least one episode of CMV viremia tended to have lower GrB B-cells as compared to patients without viremic episodes.
We demonstrate that treatment with CsA does not impair the development of GrB B-cells. GrB B-cells may have a dual role in renal transplantation as regulatory cells to maintain allospecific tolerance and as effector cells enhancing viral control.
已鉴定出产生颗粒酶B（GrB）的B细胞的一个独立亚群，其可调节T细胞介导的免疫。在本研究中，我们调查了GrB B细胞在肾移植患者（RTX）中的作用。
招募了12名健康对照（HC）和26名RTX患者。此外，还招募了19名接受环孢素A（CsA）治疗的健康志愿者。通过流式细胞术测定GrB B细胞。
与HC相比，RTX患者的GrB B细胞比例降低。健康志愿者接受CsA治疗对GrB B细胞的发育没有影响。有移植排斥史的RTX患者GrB B细胞频率增加。与没有病毒血症发作的患者相比，至少有一次巨细胞病毒血症发作的RTX患者的GrB B细胞往往较低。
我们证明CsA治疗不会损害GrB B细胞的发育。GrB B细胞在肾移植中可能具有双重作用，既作为维持同种异体特异性耐受的调节细胞，又作为增强病毒控制的效应细胞。
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