Kohlwein Sepp D
Institute of Molecular Biosciences, University of Graz, BioTechMed Graz, 8010 Graz, Austria
Cold Spring Harb Protoc. 2017 May 1;2017(5):2017/5/pdb.top078956. doi: 10.1101/pdb.top078956.
Lipids are essential biomolecules with diverse biological functions, ranging from building blocks for all biological membranes to energy substrates, signaling molecules, and protein modifiers. Despite advances in lipid analytics by mass spectrometry, the extraction and quantitative analysis of the diverse classes of lipids are still an experimental challenge. Yeast is a model organism that provides several advantages for studying lipid metabolism, because most biosynthetic pathways are well described and a great deal of information is available on the regulatory mechanisms that control lipid homeostasis. In addition, the composition of yeast lipids is much less complex than that of mammalian lipids, making yeast an excellent reference system for studying lipid-associated cell functions.
脂质是具有多种生物学功能的重要生物分子，从所有生物膜的组成成分到能量底物、信号分子和蛋白质修饰剂。尽管质谱技术在脂质分析方面取得了进展，但对不同种类脂质的提取和定量分析仍然是一项实验挑战。酵母是一种模式生物，为研究脂质代谢提供了几个优势，因为大多数生物合成途径已得到充分描述，并且有大量关于控制脂质稳态的调节机制的信息。此外，酵母脂质的组成比哺乳动物脂质的组成简单得多，这使得酵母成为研究脂质相关细胞功能的优秀参考系统。
