Fernández-Castillejo Sara, Formentín Pilar, Catalán Úrsula, Pallarès Josep, Marsal Lluís F, Solà Rosa
Unit of Lipids and Atherosclerosis Research, Department of Medicine and Surgery, Universitat Rovira I Virgili, Sant Llorenç 21, 43201 Reus, Tarragona, Spain.
Nano-electronic and Photonic Systems, Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira I Virgili, Països Catalans 26, 43007 Tarragona, Spain.
Beilstein J Nanotechnol. 2017 Mar 22;8:675-681. doi: 10.3762/bjnano.8.72. eCollection 2017.
Micro- and nanoscale substrates have been fabricated in order to study the influence of the topography on the cellular response. The aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium found in linear vessels, and to use them as substrates to study cell growth and behaviour. The influence of groove-shaped substrates on cell adhesion, morphology and proliferation were assessed, by comparing them to flat silicon substrates, used as control condition. Using human aortic endothelial cells, microscopy images demonstrate that the cellular response is different depending on the silicon surface, when it comes to cell adhesion, morphology (alignment, circularity and filopodia presence) and proliferation. Moreover, these structures exerted no cytotoxic effect. The results suggest that topographical patterning influences cell response. Silicon groove substrates can be used in developing medical devices with microscale features to mimic the endothelium in lineal vessels.
为了研究拓扑结构对细胞反应的影响，人们制备了微米级和纳米级的基底。这项工作的目的是制备不同的胶原包被硅基底，这些基底呈现出沟槽和脊状结构，以模拟线性血管中排列整齐且拉长的内皮细胞，并将它们用作研究细胞生长和行为的基底。通过将沟槽状基底与用作对照条件的平坦硅基底进行比较，评估了沟槽状基底对细胞黏附、形态和增殖的影响。使用人主动脉内皮细胞，显微镜图像表明，在细胞黏附、形态（排列、圆形度和丝状伪足的存在）和增殖方面，细胞反应因硅表面的不同而有所差异。此外，这些结构没有产生细胞毒性作用。结果表明，拓扑图案化会影响细胞反应。硅沟槽基底可用于开发具有微米级特征的医疗设备，以模拟线性血管中的内皮细胞。
