单细胞 RNA 测序揭示下丘脑前阿黑皮素原表达神经元的异质性。

Heterogeneity of hypothalamic pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencing.

机构信息

MRC Metabolic Diseases Unit, University of Cambridge Metabolic Research Laboratories, Wellcome Trust-MRC Institute of Metabolic Science, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK.

Flow Cytometry Core, Cambridge Institute of Medical Research, University of Cambridge, Cambridge CB2 0QQ, UK.

出版信息

Mol Metab. 2017 Mar 1;6(5):383-392. doi: 10.1016/j.molmet.2017.02.007. eCollection 2017 May.

OBJECTIVE

Arcuate proopiomelanocortin (POMC) neurons are critical nodes in the control of body weight. Often characterized simply as direct targets for leptin, recent data suggest a more complex architecture.

METHODS

Using single cell RNA sequencing, we have generated an atlas of gene expression in murine POMC neurons.

RESULTS

Of 163 neurons, 118 expressed high levels of with little/no expression and were considered "canonical" POMC neurons (P). The other 45/163 expressed low levels of and high levels of (AP). Unbiased clustering analysis of P neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles. Further, only 12% (14/118) of P neurons expressed the leptin receptor () compared with 58% (26/45) of AP neurons. In contrast, the insulin receptor () was expressed at similar frequency on P and AP neurons (64% and 55%, respectively).

CONCLUSION

These data reveal arcuate POMC neurons to be a highly heterogeneous population. : GSE92707.

目的

弓状核促黑皮质素原（POMC）神经元是体重控制的关键节点。这些神经元通常被简单地描述为瘦素的直接靶点，但最近的数据表明其结构更为复杂。

方法

我们使用单细胞 RNA 测序，生成了小鼠 POMC 神经元基因表达图谱。

结果

在 163 个神经元中，有 118 个神经元高水平表达，而几乎没有/没有表达，被认为是“经典”的 POMC 神经元（P 型）。其余 45/163 个神经元低水平表达，高水平表达（AP 型）。对 P 型神经元进行无偏聚类分析，揭示了四个不同的细胞群，每个细胞群都具有独特的细胞表面受体基因表达谱。此外，只有 12%（14/118）的 P 型神经元表达瘦素受体（），而 58%（26/45）的 AP 型神经元表达。相比之下，胰岛素受体（）在 P 型和 AP 型神经元上的表达频率相似（分别为 64%和 55%）。