Youm Jae Boum
National Research Laboratory for Mitochondrial Signaling Laboratory, Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University, Busan, Korea.
Integr Med Res. 2016 Mar;5(1):3-10. doi: 10.1016/j.imr.2015.12.007. Epub 2016 Jan 7.
Embryonic stem cell-derived cardiomyocytes (ESC-CMs) hold great interest in many fields of research including clinical applications such as stem cell and gene therapy for cardiac repair or regeneration. ESC-CMs are also used as a platform tool for pharmacological tests or for investigations of cardiac remodeling. ESC-CMs have many different aspects of morphology, electrophysiology, calcium handling, and bioenergetics compared with adult cardiomyocytes. They are immature in morphology, similar to sinus nodal-like in the electrophysiology, higher contribution of trans-sarcolemmal Ca influx to Ca handling, and higher dependence on anaerobic glycolysis. Here, I review a detailed electrophysiology and Ca handling features of ESC-CMs during differentiation into adult cardiomyocytes to gain insights into how all the developmental changes are related to each other to display cardinal features of developing cardiomyocytes.
胚胎干细胞衍生的心肌细胞（ESC-CMs）在许多研究领域都备受关注，包括临床应用，如用于心脏修复或再生的干细胞和基因治疗。ESC-CMs还被用作药理测试或心脏重塑研究的平台工具。与成年心肌细胞相比，ESC-CMs在形态、电生理学、钙处理和生物能量学方面有许多不同之处。它们在形态上不成熟，电生理学上类似于窦房结样，跨肌膜钙内流对钙处理的贡献更高，并且对无氧糖酵解的依赖性更高。在此，我回顾了ESC-CMs在分化为成年心肌细胞过程中的详细电生理学和钙处理特征，以深入了解所有发育变化是如何相互关联的，从而展现发育中心肌细胞的主要特征。
