利用产丙酮酸的大肠杆菌菌株生产d-乳酸。
Production of d-lactate using a pyruvate-producing Escherichia coli strain.
作者信息
Akita Hironaga, Nakashima Nobutaka, Hoshino Tamotsu
机构信息
a Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science Technology (AIST) , Higashi-Hiroshima , Japan.
b Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , Sapporo , Japan.
出版信息
Biosci Biotechnol Biochem. 2017 Jul;81(7):1452-1455. doi: 10.1080/09168451.2017.1318697. Epub 2017 May 2.
To generate an organism capable of producing d-lactate, NAD-dependent d-lactate dehydrogenase was expressed in our pyruvate-producing strain, Escherichia coli strain LAFCPCPt-accBC-aceE. After determining the optimal culture conditions for d-lactate production, 18.4 mM d-lactate was produced from biomass-based medium without supplemental mineral or nitrogen sources. Our results show that d-lactate can be produced in simple batch fermentation processes.
为了构建一种能够产生d-乳酸的生物体，在我们的丙酮酸生产菌株大肠杆菌LAFCPCPt-accBC-aceE中表达了NAD依赖性d-乳酸脱氢酶。在确定了d-乳酸生产的最佳培养条件后，在不添加矿物质或氮源的基于生物质的培养基中产生了18.4 mM的d-乳酸。我们的结果表明，d-乳酸可以在简单的分批发酵过程中产生。
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