Kimura H, Nakano M
College of Medical Care and Technology, Gunma University, Japan.
FEBS Lett. 1988 Nov 7;239(2):347-50. doi: 10.1016/0014-5793(88)80949-9.
Superoxide dismutase in human erythrocytes was assayed by the inhibition of highly diluted erythrocyte lysates on 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazol[1,2-alpha]pyra zin-3-one- dependent luminescence induced by the hypoxanthine-xanthine oxidase system. Our chemiluminescence procedure gave 95-times higher sensitivity than the cytochrome c method. The concentration of superoxide dismutase in erythrocytes of Down's syndrome patients was approx. 1.8-times higher than that of normal humans.
通过检测高度稀释的红细胞裂解物对次黄嘌呤-黄嘌呤氧化酶系统诱导的2-甲基-6-（对甲氧基苯基）-3,7-二氢咪唑并[1,2-α]吡嗪-3-酮依赖性发光的抑制作用，来测定人红细胞中的超氧化物歧化酶。我们的化学发光法比细胞色素c法的灵敏度高95倍。唐氏综合征患者红细胞中超氧化物歧化酶的浓度约比正常人高1.8倍。
