Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai, China; Forschungszentrum Jülich GmbH, Agrosphere (IBG-3), Jülich 52428, Germany.
Laboratory of Environmental Remediation, College of Environmental and Chemical Engineering, Shanghai University, Shanghai, China.
Sci Total Environ. 2017 Dec 1;599-600:50-57. doi: 10.1016/j.scitotenv.2017.04.083. Epub 2017 Apr 29.
Though phytoremediation is deemed as a promising approach to restore polycyclic aromatic hydrocarbon (PAHs) contaminated sites, studies about how the biodegradation of PAHs is enhanced still remains incomprehensive. Effects of root components on pyrene bioaccessibility, soil enzymes and microbial communities were explored in the paper, and their interactions in simulated pyrene and pyrene-diesel spiked microcosms were tried to give a reasonable explanation. Results indicated that root components enhanced the pyrene removal of bioaccessible and adsorbed fractions by 16.10 and 1.80mgkg, respectively, in pyrene-spiked soils at the end of the experiment. By contrast, root components increased the degradation of bioaccessible fraction by only 3.3mgkg in pyrene-diesel spiked soils. Although the bound fractions of pyrene increased over time in treatments without root components, they remained relatively stable, ranging from 0.02 to 0.03mgkg, in root components amended treatments. Activities of soil enzymes (polyphenol oxidase, catalase, invertase, urease and alkaline phosphatase) varied differently in response to pollutants and root components. Analysis of phospholipid fatty acids revealed that root components increased the biomass of soil microorganisms and altered the microbial structure. Pearson correlation analysis proved positive correlations between all the microbial subgroups and pyrene removal in pyrene-spiked soils, but the degradation of bioaccessible pyrene was only positively related with microorganisms confirmed by monounsaturated fatty acids in pyrene-diesel spiked soils.
尽管植物修复被认为是一种很有前途的方法，可以修复多环芳烃（PAHs）污染的场地，但关于如何增强 PAHs 生物降解的研究仍然不够全面。本文探讨了根成分对芘生物可利用性、土壤酶和微生物群落的影响，并试图解释它们在模拟芘和芘-柴油污染微宇宙中的相互作用。结果表明，在实验结束时，根成分分别将可生物利用和吸附部分的芘去除率提高了 16.10 和 1.80mgkg。相比之下，根成分仅使可生物利用部分的降解增加了 3.3mgkg。虽然在没有根成分的处理中，结合态的芘随着时间的推移而增加，但在根成分处理中，它们仍然保持相对稳定，范围在 0.02 到 0.03mgkg 之间。土壤酶（多酚氧化酶、过氧化氢酶、转化酶、脲酶和碱性磷酸酶）的活性对污染物和根成分的反应不同。磷脂脂肪酸分析表明，根成分增加了土壤微生物的生物量并改变了微生物结构。Pearson 相关分析证明，在芘污染土壤中，所有微生物亚群与芘去除之间均存在正相关关系，但在芘-柴油污染土壤中，可生物利用芘的降解仅与单不饱和脂肪酸鉴定的微生物呈正相关。
