Lerivray H, Smith J A, Tata J R
Laboratory of Developmental Biochemistry, National Institute for Medical Research, Mill Hill, London, U.K.
Mol Cell Endocrinol. 1988 Oct;59(3):241-8. doi: 10.1016/0303-7207(88)90109-8.
We describe the isolation of a cloned cDNA from a cDNA library of oviduct of estrogen-treated adult Xenopus. Although the protein encoded by this cDNA is not known, it is designated as FOSP-1 (frog oviduct-specific protein-1). A partial restriction map of FOSP-1 cDNA, which is 1.5 kb in size, is presented. Northern hybridization analysis showed that FOSP-1 cDNA codes for a single species of mRNA of 2.6 kb which is exclusively expressed in Xenopus oviduct. Southern blot analysis showed that the gene was present in only one or two copies. Sequencing of partial FOSP-1 cDNA did not reveal homology with any protein in the sequence data bank. Measurement of steady-state levels of FOSP-1 mRNA in primary cultures of Xenopus oviduct cells by a technique of quantitative slot-blot analysis showed that both 17 beta and 17 alpha stereoisomers of estradiol caused a rapid 5-fold enhancement of accumulation of the mRNA with maximum values obtained at 5 X 10(-8) M estrogen. Progesterone caused only a small increase in FOSP-1 mRNA concentration. This hormone-specific induction of mRNA makes FOSP-1 a valuable candidate for exploring tissue specificity of regulation by estrogen of gene expression.
我们描述了从经雌激素处理的成年非洲爪蟾输卵管cDNA文库中分离出一个克隆的cDNA。尽管该cDNA编码的蛋白质尚不清楚，但将其命名为FOSP-1（蛙输卵管特异性蛋白-1）。给出了大小为1.5 kb的FOSP-1 cDNA的部分限制性图谱。Northern杂交分析表明，FOSP-1 cDNA编码一种2.6 kb的单一mRNA，该mRNA仅在非洲爪蟾输卵管中表达。Southern印迹分析表明该基因仅以单拷贝或双拷贝存在。FOSP-1 cDNA部分序列的测序未显示与序列数据库中的任何蛋白质具有同源性。通过定量狭缝印迹分析技术测量非洲爪蟾输卵管细胞原代培养物中FOSP-1 mRNA的稳态水平，结果表明雌二醇的17β和17α立体异构体均能使mRNA积累迅速增加5倍，在5×10⁻⁸ M雌激素时达到最大值。孕酮仅使FOSP-1 mRNA浓度略有增加。这种mRNA的激素特异性诱导使得FOSP-1成为探索雌激素对基因表达调控的组织特异性的有价值候选物。
