Stainsby W N, Eitzman P D
Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
J Appl Physiol (1985). 1988 Oct;65(4):1803-10. doi: 10.1152/jappl.1988.65.4.1803.
To determine the origins of the arteriovenous [H+] difference of muscle during contractions, arterial and muscle venous blood sample pairs were taken before and after 0.5, 5.0, and 30.0 min of 4/s isometric twitches of the gastrocnemius-plantaris muscle group of anesthetized dogs. These samples were analyzed for PO2, PCO2, and pH, the concentrations of O2, CO2, K+, Na+, La-, and Cl- in whole blood, and La-, K+, Na+, and Cl- in plasma. Whole blood was hemolyzed and analyzed for PO2, PCO2, and pH. Net O2 uptake, CO2 output, L, K+, Na+, and Cl- were calculated in addition to net output of non-CO2 acid (HA) and strong ion difference ([SID]) and common ion [SID] ([K+] + [Na+] - [Cl-] - [La-]). From these data we partitioned the origins of the arteriovenous [H+] difference via the common PCO2-pH diagram and via a [H+]-PCO2 diagram and determined whether true plasma arteriovenous [H+] differences reflect plasma and cell arteriovenous [H+] differences. The arteriovenous [H+] differences of plasma and hemolyzed blood were the same, showing that true plasma does reflect plasma and cells. K+ showed a small significant but transient output. Na+ was not significant, whereas Cl- showed a significant transient uptake. Lactate output and HA, calculated for dog blood acid-base, showed transient outputs and were the same. At 5.0 min when the arteriovenous difference was largest, CO2 alone would have increased [H+] 15.9 nmol/l whereas desaturation of Hb would have decreased [H+] 4.2 nmol/l and lactate could have raised [H+] 1.0 nmol/l.(ABSTRACT TRUNCATED AT 250 WORDS)
为确定肌肉收缩期间动静脉[H⁺]差异的来源，在麻醉犬的腓肠肌 - 跖肌组以4次/秒的频率进行0.5、5.0和30.0分钟等长收缩前后，采集动脉血和肌肉静脉血样本对。对这些样本进行了PO₂、PCO₂和pH分析，以及全血中O₂、CO₂、K⁺、Na⁺、乳酸根和Cl⁻的浓度，还有血浆中乳酸根、K⁺、Na⁺和Cl⁻的浓度分析。将全血进行溶血后分析PO₂、PCO₂和pH。除了计算非CO₂酸（HA）和强离子差（[SID]）以及共同离子[SID]（[K⁺] + [Na⁺] - [Cl⁻] - [乳酸根]）的净输出外，还计算了净O₂摄取、CO₂输出、L、K⁺、Na⁺和Cl⁻。根据这些数据，我们通过共同的PCO₂ - pH图和[H⁺] - PCO₂图划分了动静脉[H⁺]差异的来源，并确定真正的血浆动静脉[H⁺]差异是否反映血浆和细胞的动静脉[H⁺]差异。血浆和溶血血液的动静脉[H⁺]差异相同，表明真正的血浆确实反映了血浆和细胞的情况。K⁺显示出少量显著但短暂的输出。Na⁺不显著，而Cl⁻显示出显著的短暂摄取。针对犬类血液酸碱计算的乳酸输出和HA显示出短暂输出且相同。在动静脉差异最大的5.0分钟时，仅CO₂会使[H⁺]增加15.9 nmol/L，而血红蛋白去饱和会使[H⁺]降低4.2 nmol/L，乳酸可使[H⁺]升高1.0 nmol/L。（摘要截取自250字）
