• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

两种钻蛀性内寄生蜂（小菜蛾绒茧蜂和半闭弯尾姬蜂）毒腺的比较转录组分析。

Comparative transcriptome analysis of venom glands from Cotesia vestalis and Diadromus collaris, two endoparasitoids of the host Plutella xylostella.

机构信息

Institute of Insect Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.

Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.

出版信息

Sci Rep. 2017 May 2;7(1):1298. doi: 10.1038/s41598-017-01383-2.

DOI:10.1038/s41598-017-01383-2
PMID:28465546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5431001/
Abstract

Venoms secreted by the venom gland (VG) of parasitoid wasp help ensure successful parasitism by host immune suppression and developmental regulation. Cotesia vestalis, a larval endoparasitoid, and Diadromus collaris, a pupal endoparasitoid, parasitize the diamondback moth (DBM), Plutella xylostella. To explore and compare the venom components of two endoparasitoids, we sequenced transcriptomes of the VGs and wasp bodies without VGs (BWVGs) of the two endoparasitoids. Statistically enriched GO terms and KEGG pathways of the two VGs compared to respective whole-body background were similar and reflected active protein biosynthesis activities in the two VGs. 1,595 VG specific genes of the D. collaris VG and 1,461 VG specific genes of the C. vestalis VG were identified by comparative transcript profiling. A total of 444 and 513 genes encoding potential secretory proteins were identified and defined as putative venom genes in D. collaris VG and C. vestalis VG, respectively. The putative venom genes of the two wasps showed no significant similarity or convergence. More venom genes were predicted in D. collaris VG than C. vestalis VG, especially hydrolase-coding genes. Differences in the types and quantities of putative venom genes shed light on different venom functions.

摘要

蜂的毒液腺（VG）分泌的毒液有助于通过宿主免疫抑制和发育调节来确保成功寄生。小菜蛾幼虫内寄生蜂，甘蓝夜蛾幼虫内寄生蜂，和钻蛀性蛹内寄生蜂，Diadromus collaris，寄生小菜蛾，Plutella xylostella。为了探索和比较两种内寄生蜂的毒液成分，我们对两种内寄生蜂的 VG 和无 VG 的蜂体（BWVG）进行了转录组测序。与各自的全身背景相比，两种 VG 的统计上富集的 GO 术语和 KEGG 途径相似，反映了两种 VG 中活跃的蛋白质生物合成活性。通过比较转录谱，鉴定了 1595 个 D. collaris VG 的 VG 特异性基因和 1461 个 C. vestalis VG 的 VG 特异性基因。总共鉴定出 444 个和 513 个编码潜在分泌蛋白的基因，并分别将其定义为 D. collaris VG 和 C. vestalis VG 中的假定毒液基因。两种蜂的假定毒液基因之间没有明显的相似性或趋同。与 C. vestalis VG 相比，D. collaris VG 预测出更多的毒液基因，尤其是水解酶编码基因。假定毒液基因的类型和数量的差异揭示了不同的毒液功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/05107c0b4156/41598_2017_1383_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/2364263e2810/41598_2017_1383_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/910a34111efa/41598_2017_1383_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/363f4c5d3460/41598_2017_1383_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/feb4e5f7b816/41598_2017_1383_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/baa8d93da387/41598_2017_1383_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/05107c0b4156/41598_2017_1383_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/2364263e2810/41598_2017_1383_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/910a34111efa/41598_2017_1383_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/363f4c5d3460/41598_2017_1383_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/feb4e5f7b816/41598_2017_1383_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/baa8d93da387/41598_2017_1383_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc5/5431001/05107c0b4156/41598_2017_1383_Fig6_HTML.jpg

相似文献

1
Comparative transcriptome analysis of venom glands from Cotesia vestalis and Diadromus collaris, two endoparasitoids of the host Plutella xylostella.两种钻蛀性内寄生蜂（小菜蛾绒茧蜂和半闭弯尾姬蜂）毒腺的比较转录组分析。
Sci Rep. 2017 May 2;7(1):1298. doi: 10.1038/s41598-017-01383-2.
2
Characterization of larval gut microbiota of two endoparasitoid wasps associated with their common host, (Linnaeus) (Lepidoptera: Plutellidae).两种与共同宿主（鳞翅目：菜粉蝶科）相关的内寄生蜂幼虫肠道微生物群落特征。
Microbiol Spectr. 2024 Oct 3;12(10):e0120824. doi: 10.1128/spectrum.01208-24. Epub 2024 Sep 9.
3
The genomes of two parasitic wasps that parasitize the diamondback moth.寄生在小菜蛾上的两种寄生蜂的基因组。
BMC Genomics. 2019 Nov 21;20(1):893. doi: 10.1186/s12864-019-6266-0.
4
Interspecific competition between two endoparasitoids Cotesia vestalis (Hymenoptera: Braconidae) and Oomyzus sokolowskii (Hymenoptera: Eulophidae).两种内寄生蜂， cioè Cotesia vestalis（膜翅目：Braconidae）和 Oomyzus sokolowskii（膜翅目：Eulophidae）之间的种间竞争。
Arch Insect Biochem Physiol. 2011 Mar;76(3):156-67. doi: 10.1002/arch.20399. Epub 2010 Dec 6.
5
Biological study of Plutella xylostella (L.) (Lep: Plutellidae) and it's solitary endoparasitoid, Cotesia vestalis (Haliday) (Hym. Braconidae) under laboratory conditions.小菜蛾（鳞翅目：菜蛾科）及其单寄生内寄生蜂菜蛾盘绒茧蜂（膜翅目：茧蜂科）在实验室条件下的生物学研究
Pak J Biol Sci. 2011 Dec 15;14(24):1090-9. doi: 10.3923/pjbs.2011.1090.1099.
6
Insights into the venom composition and evolution of an endoparasitoid wasp by combining proteomic and transcriptomic analyses.通过蛋白质组学和转录组学分析深入了解一种内寄生蜂的毒液成分和进化
Sci Rep. 2016 Jan 25;6:19604. doi: 10.1038/srep19604.
7
Partial venom gland transcriptome of a Drosophila parasitoid wasp, Leptopilina heterotoma, reveals novel and shared bioactive profiles with stinging Hymenoptera.异色瓢虫柄腹姬小蜂部分毒液腺转录组分析揭示其与螫刺膜翅目昆虫具有新颖且共享的生物活性特征。
Gene. 2013 Sep 10;526(2):195-204. doi: 10.1016/j.gene.2013.04.080. Epub 2013 May 17.
8
Parasitic castration of Plutella xylostella larvae induced by polydnaviruses and venom of Cotesia vestalis and Diadegma semiclausum.由多角体病毒以及小菜蛾绒茧蜂和半闭弯尾姬蜂的毒液诱导的小菜蛾幼虫的寄生性阉割。
Arch Insect Biochem Physiol. 2009 Jan;70(1):30-43. doi: 10.1002/arch.20279.
9
Identification of floral volatiles from Fagopyrum esculentum that attract Cotesia vestalis with potentially better biocontrol efficacy against Plutella xylostella.鉴定苦荞中能吸引丽蚜小蜂的花香挥发物，以期对小菜蛾有更好的生物防治效果。
Pest Manag Sci. 2024 Feb;80(2):763-775. doi: 10.1002/ps.7808. Epub 2023 Oct 11.
10
A teratocyte-specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase-activating system of its host Plutella xylostella.一种源自内寄生蜂小茧蜂的滋养细胞特异性丝氨酸蛋白酶抑制剂抑制了其宿主小菜蛾的酚氧化酶原激活系统。
Insect Mol Biol. 2022 Apr;31(2):202-215. doi: 10.1111/imb.12751. Epub 2021 Dec 28.

引用本文的文献

1
A rare, evolutionarily conserved venom protein benefits endoparasitism across parasitoids.一种罕见的、进化上保守的毒液蛋白有利于各类寄生蜂的体内寄生。
Cell Genom. 2025 Aug 13;5(8):100920. doi: 10.1016/j.xgen.2025.100920. Epub 2025 Jun 19.
2
Integration of transcriptomic and proteomic data uncovers the nutritional requirement of the immature development of an endoparasitoid wasp.转录组学和蛋白质组学数据的整合揭示了一种内寄生蜂未成熟发育的营养需求。
Sci Rep. 2025 May 22;15(1):17744. doi: 10.1038/s41598-025-02290-7.
3
Multi-omic approach to characterize the venom of the parasitic wasp Cotesia congregata (Hymenoptera: Braconidae).

本文引用的文献

1
Insights into function and evolution of parasitoid wasp venoms.对寄生蜂毒液功能与进化的见解。
Curr Opin Insect Sci. 2014 Dec;6:52-60. doi: 10.1016/j.cois.2014.10.004. Epub 2014 Oct 25.
2
Insights into the venom composition and evolution of an endoparasitoid wasp by combining proteomic and transcriptomic analyses.通过蛋白质组学和转录组学分析深入了解一种内寄生蜂的毒液成分和进化
Sci Rep. 2016 Jan 25;6:19604. doi: 10.1038/srep19604.
3
The Pfam protein families database: towards a more sustainable future.Pfam蛋白质家族数据库：迈向更可持续的未来。
采用多组学方法表征寄生黄蜂聚集茧蜂（膜翅目：茧蜂科）的毒液。
BMC Genomics. 2025 Apr 30;26(1):431. doi: 10.1186/s12864-025-11604-y.
4
Symbiotic bracovirus of a parasite modulate host ecdysis process.一种寄生虫的共生杆状病毒可调节宿主的蜕皮过程。
Cell Mol Life Sci. 2025 Apr 28;82(1):183. doi: 10.1007/s00018-025-05717-6.
5
Characterization of larval gut microbiota of two endoparasitoid wasps associated with their common host, (Linnaeus) (Lepidoptera: Plutellidae).两种与共同宿主（鳞翅目：菜粉蝶科）相关的内寄生蜂幼虫肠道微生物群落特征。
Microbiol Spectr. 2024 Oct 3;12(10):e0120824. doi: 10.1128/spectrum.01208-24. Epub 2024 Sep 9.
6
Parasitoid Serpins Evolve Novel Functions to Manipulate Host Homeostasis.寄生性丝氨酸蛋白酶抑制剂通过进化获得操纵宿主内稳态的新功能。
Mol Biol Evol. 2023 Dec 1;40(12). doi: 10.1093/molbev/msad269.
7
A serpin gene from a parasitoid wasp disrupts host immunity and exhibits adaptive alternative splicing.一种寄生蜂的丝氨酸蛋白酶抑制剂基因可破坏宿主免疫，并表现出适应性的可变剪接。
PLoS Pathog. 2023 Sep 11;19(9):e1011649. doi: 10.1371/journal.ppat.1011649. eCollection 2023 Sep.
8
Mining Amphibian and Insect Transcriptomes for Antimicrobial Peptide Sequences with rAMPage.利用rAMPage挖掘两栖动物和昆虫转录组中的抗菌肽序列。
Antibiotics (Basel). 2022 Jul 15;11(7):952. doi: 10.3390/antibiotics11070952.
9
A teratocyte-specific serpin from the endoparasitoid wasp Cotesia vestalis inhibits the prophenoloxidase-activating system of its host Plutella xylostella.一种源自内寄生蜂小茧蜂的滋养细胞特异性丝氨酸蛋白酶抑制剂抑制了其宿主小菜蛾的酚氧化酶原激活系统。
Insect Mol Biol. 2022 Apr;31(2):202-215. doi: 10.1111/imb.12751. Epub 2021 Dec 28.
10
Comparative Transcriptome Analysis Reveals Sex-Based Differences during the Development of the Adult Parasitic Wasp (Hymenoptera: Braconidae).比较转录组分析揭示了成年寄生蜂（膜翅目：Braconidae）发育过程中的性别差异。
Genes (Basel). 2021 Jun 10;12(6):896. doi: 10.3390/genes12060896.
Nucleic Acids Res. 2016 Jan 4;44(D1):D279-85. doi: 10.1093/nar/gkv1344. Epub 2015 Dec 15.
4
A Venom Gland Extracellular Chitin-Binding-Like Protein from Pupal Endoparasitoid Wasps, Pteromalus Puparum, Selectively Binds Chitin.来自蛹期内寄生蜂蝶蛹金小蜂的一种毒液腺细胞外类几丁质结合蛋白可选择性结合几丁质。
Toxins (Basel). 2015 Nov 30;7(12):5098-113. doi: 10.3390/toxins7124867.
5
HMMER web server: 2015 update.HMMER网络服务器：2015年更新版。
Nucleic Acids Res. 2015 Jul 1;43(W1):W30-8. doi: 10.1093/nar/gkv397. Epub 2015 May 5.
6
Uncovering the novel characteristics of Asian honey bee, Apis cerana, by whole genome sequencing.通过全基因组测序揭示亚洲蜜蜂（东方蜜蜂）的新特征。
BMC Genomics. 2015 Jan 2;16(1):1. doi: 10.1186/1471-2164-16-1.
7
De Novo sequencing and transcriptome analysis for Tetramorium bicarinatum: a comprehensive venom gland transcriptome analysis from an ant species.双隆骨铺道蚁的从头测序与转录组分析：来自一种蚂蚁物种的全面毒腺转录组分析
BMC Genomics. 2014 Nov 18;15(1):987. doi: 10.1186/1471-2164-15-987.
8
Systematic analysis of a wasp parasitism arsenal.系统分析一种黄蜂寄生武器库。
Mol Ecol. 2014 Feb;23(4):890-901. doi: 10.1111/mec.12648.
9
Integrative approach reveals composition of endoparasitoid wasp venoms.综合方法揭示内寄生蜂毒液的组成。
PLoS One. 2013 May 23;8(5):e64125. doi: 10.1371/journal.pone.0064125. Print 2013.
10
Extensive inter- and intraspecific venom variation in closely related parasites targeting the same host: the case of Leptopilina parasitoids of Drosophila.密切相关的寄生虫针对同一宿主的毒液存在广泛的种间和种内变异：以果蝇的寄生蜂 Leptopilina 为例。
Insect Biochem Mol Biol. 2013 Jul;43(7):601-11. doi: 10.1016/j.ibmb.2013.03.010. Epub 2013 Apr 2.