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转基因甘薯中类胡萝卜素的代谢工程

Metabolic engineering of carotenoids in transgenic sweetpotato.

作者信息

Kang Le, Park Sung-Chul, Ji Chang Yoon, Kim Ho Soo, Lee Haeng-Soon, Kwak Sang-Soo

机构信息

Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.

Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), Daejeon 305-350, Republic of Korea.

出版信息

Breed Sci. 2017 Jan;67(1):27-34. doi: 10.1270/jsbbs.16118. Epub 2017 Feb 17.

DOI:10.1270/jsbbs.16118
PMID:28465665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5407916/
Abstract

Sweetpotato [ (L.) Lam], which contains high levels of antioxidants such as ascorbate and carotenoids in its storage root, is one of the healthiest foods, as well as one of the best starch crops for growth on marginal lands. In plants, carotenoid pigments are involved in light harvesting for photosynthesis and are also essential for photo-protection against excess light. As dietary antioxidants in humans, these compounds benefit health by alleviating aging-related diseases. The storage root of sweetpotato is a good source of both carotenoids and carbohydrates for human consumption. Therefore, metabolic engineering of sweetpotato to increase the content of useful carotenoids represents an important agricultural goal. This effort has been facilitated by cloning of most of the carotenoid biosynthetic genes, as well as the gene involved in carotenoid accumulation. In this review, we describe our current understanding of the regulation of biosynthesis, accumulation and catabolism of carotenoids in sweetpotato. A deeper understanding of these topics should contribute to development of new sweetpotato cultivars with higher levels of nutritional carotenoids and abiotic stress tolerance.

摘要

甘薯[（L.）Lam]在其块根中含有高水平的抗氧化剂，如抗坏血酸和类胡萝卜素，它是最健康的食物之一，也是在边际土地上生长的最佳淀粉作物之一。在植物中，类胡萝卜素色素参与光合作用的光捕获，并且对于防止过量光照的光保护也至关重要。作为人类饮食中的抗氧化剂，这些化合物通过减轻与衰老相关的疾病而有益于健康。甘薯块根是人类食用类胡萝卜素和碳水化合物的良好来源。因此，通过代谢工程提高甘薯中有用类胡萝卜素的含量是一个重要的农业目标。大多数类胡萝卜素生物合成基因以及参与类胡萝卜素积累的基因的克隆促进了这一努力。在这篇综述中，我们描述了目前对甘薯中类胡萝卜素生物合成、积累和分解代谢调控的理解。对这些主题的更深入理解应该有助于开发具有更高营养类胡萝卜素水平和非生物胁迫耐受性的新甘薯品种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ce/5407916/b62b64aa6ec3/67_16118_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ce/5407916/2f87b8450d01/67_16118_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ce/5407916/beb013764c23/67_16118_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ce/5407916/b62b64aa6ec3/67_16118_3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ce/5407916/2f87b8450d01/67_16118_1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ce/5407916/beb013764c23/67_16118_2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2ce/5407916/b62b64aa6ec3/67_16118_3.jpg

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本文引用的文献

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Sci Rep. 2016 Sep 16;6:33563. doi: 10.1038/srep33563.
2
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C R Biol. 2016 May-Jun;339(5-6):207-213. doi: 10.1016/j.crvi.2016.04.010.
3
Molecular characterization of tocopherol biosynthetic genes in sweetpotato that respond to stress and activate the tocopherol production in tobacco.
转录因子IbNAC29正向调控甘薯中类胡萝卜素的积累。
Hortic Res. 2023 Feb 1;10(3):uhad010. doi: 10.1093/hr/uhad010. eCollection 2023 Mar.
4
Chemistry, Occurrence, Properties, Applications, and Encapsulation of Carotenoids-A Review.类胡萝卜素的化学、存在、性质、应用及包封——综述
Plants (Basel). 2023 Jan 9;12(2):313. doi: 10.3390/plants12020313.
5
Integrated Metabolomic and Transcriptomic Analyses Reveal the Basis for Carotenoid Biosynthesis in Sweet Potato ( (L.) Lam.) Storage Roots.综合代谢组学和转录组学分析揭示甘薯（Ipomoea batatas (L.) Lam.）块根中类胡萝卜素生物合成的基础。
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