Langier Sheila, Benor Shira, Kadar Laliv, Shani Nir, Etkin Sara, Stark Moshe, Bondar Ekaterina, Aizic Asaf, Kivity Shmuel
Allergy and Immunology Unit, Tel Aviv Medical Center, Tel Aviv, Israel.
Microsurgery and Plastic Surgery Laboratory of the Plastic Surgery Department, Tel Aviv Medical Center, Tel Aviv, Israel.
Clin Exp Pharmacol Physiol. 2017 Dec;44 Suppl 1:64-69. doi: 10.1111/1440-1681.12778. Epub 2017 Oct 19.
Aging is associated with altered decreased barrier function in the skin, which can lead to different types of immunoglobulin E (IgE)-mediated sensitization to environmental allergens. Yet, allergen-specific respiratory sensitization among the elderly is not well described. The aim of this study was to investigate the effect of aging on allergic pulmonary inflammation induced by epicutaneous sensitization of mechanically irritated skin in mice. For this purpose, 6-week-, 6-month-, and 18-month-old female BALB/c mice, underwent epicutaneous sensitization with ovalbumin (OVA) or phosphate buffered saline (PBS), followed by an inhaled OVA challenge. Blood OVA-specific IgE levels measured after epicutaneous sensitization, as well as, bronchial alveolar lavage fluids (BALF) leucocyte, eosinophil, and cytokine levels measured after OVA inhalation challenge were similar among the 6-week-old (young) and 6-month-old (adult) groups. However, significantly decreased levels of systemic OVA IgE, and BALF leukocyte, eosinophil and T helper cell type 2 cytokine levels, were measured after OVA inhalation challenge in elderly (18-month-old) mice compared to the other groups of mice. In addition, interleukin-10 (IL-10), a regulatory suppressor cytokine, was more abundant in the BALF of the elderly group after epicutaneous sensitization and inhalation challenge. Our results suggest that elderly mice have a reduced allergic response to induced sensitization with OVA, possibly regulated by increased IL-10 levels.
衰老与皮肤屏障功能改变及降低有关，这可能导致不同类型的免疫球蛋白E（IgE）介导的对环境过敏原的致敏。然而，老年人中过敏原特异性呼吸道致敏情况尚未得到充分描述。本研究的目的是调查衰老对小鼠机械刺激皮肤经皮致敏诱导的过敏性肺部炎症的影响。为此，对6周龄、6月龄和18月龄的雌性BALB/c小鼠用卵清蛋白（OVA）或磷酸盐缓冲盐水（PBS）进行经皮致敏，随后进行OVA吸入激发。经皮致敏后测得的血液OVA特异性IgE水平，以及OVA吸入激发后测得的支气管肺泡灌洗液（BALF）中的白细胞、嗜酸性粒细胞和细胞因子水平，在6周龄（年轻）和6月龄（成年）组中相似。然而，与其他组小鼠相比，老年（18月龄）小鼠在OVA吸入激发后，全身OVA IgE水平以及BALF中的白细胞、嗜酸性粒细胞和2型辅助性T细胞细胞因子水平显著降低。此外，在经皮致敏和吸入激发后，调节性抑制细胞因子白细胞介素-10（IL-10）在老年组的BALF中含量更高。我们的结果表明，老年小鼠对OVA诱导的致敏过敏反应降低，可能是由IL-10水平升高调节的。
