Kulkarni P S, Kaufman P L, Srinivasan B D
Department of Pharmacology, Columbia University, New York, New York.
J Ocul Pharmacol. 1987 Winter;3(4):349-56. doi: 10.1089/jop.1987.3.349.
We have demonstrated that cynomolgus and rhesus monkey ocular tissues such as conjunctiva and eyelids have the capacity to synthesize cyclo-oxygenase and lipoxygenase products from arachidonic acid (AA). In the present study, we demonstrate that another fatty acid, eicosapentaenoic acid (C20:5), which is found mainly in marine diets, can be converted by cynomolgus and rhesus monkey conjunctiva and eyelids into cyclo-oxygenase (3-series) and lipoxygenase (5-series) products. EPA-lipoxygenase activity (ranging from 6.5% to 10.4%) was greater than EPA-cyclo-oxygenase activity (ranging from 1.5% to 3.5%) in these tissues. Furthermore, BW755, a cyclo-oxygenase and lipoxygenase inhibitor, inhibited the synthesis of EPA-derived cyclo-oxygenase and lipoxygenase product synthesis, indicating the enzymatic nature of EPA metabolism.
我们已经证明，食蟹猴和恒河猴的眼组织，如结膜和眼睑，有能力从花生四烯酸（AA）合成环氧化酶和脂氧化酶产物。在本研究中，我们证明另一种主要存在于海洋饮食中的脂肪酸二十碳五烯酸（C20:5），可被食蟹猴和恒河猴的结膜和眼睑转化为环氧化酶（3-系列）和脂氧化酶（5-系列）产物。在这些组织中，EPA-脂氧化酶活性（范围为6.5%至10.4%）大于EPA-环氧化酶活性（范围为1.5%至3.5%）。此外，环氧化酶和脂氧化酶抑制剂BW755抑制了EPA衍生的环氧化酶和脂氧化酶产物的合成，表明EPA代谢具有酶促性质。
