皮肤接触煤焦油的儿童中抗苯并[a]芘-7,8-二醇-9,10-环氧化物-DNA加合物抗体（IgG、IgM）的血清水平

Serum Level of Antibodies (IgG, IgM) Against Benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA Adducts in Children Dermatologically Exposed to Coal Tar.

作者信息

Borský Pavel, Andrýs Ctirad, Krejsek Jan, Hamáková Květoslava, Kremláček Jan, Málková Andrea, Bartošová Lenka, Fiala Zdeněk, Palička Vladimír, Borská Lenka

机构信息

Institute of Hygiene and Preventive Medicine, Charles University, Faculty of Medicine, Hradec Králové, Czech Republic.

Institute of Clinical Immunology and Allergology, Charles University, Faculty of Medicine, Hradec Králové, Czech Republic.

出版信息

Acta Medica (Hradec Kralove). 2017;60(1):27-31. doi: 10.14712/18059694.2017.46. Epub 2017 May 3.

DOI:10.14712/18059694.2017.46

PMID:28467299

Abstract

Crude coal tar (CCT) contains polycyclic aromatic hydrocarbons (PAHs). Benzo[a]pyrene (BaP) is metabolized into a highly reactive metabolite benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) that is able to bind to DNA and creates BPDE-DNA adducts. Adducted DNA becomes immunogenic and induces immune response by production of antibodies against BPDE-DNA adducts (Ab-BPDE-DNA). Circulating Ab-BPDE-DNA was proposed as potential biomarker of genotoxic exposure to BaP (PAHs). Goeckerman therapy (GT) of psoriasis uses dermal application of CCT ointment (PAHs). In presented study (children with psoriasis treated by GT; n = 19) the therapy significantly increased the level of Ab-BPDE-DNA (EI = 0.29/0.19-0.34 vs. 0.31/0.25-0.40; median/lower-upper quartile; p < 0.01). The results support the idea of Ab-BPDE-DNA level as a possible tentative indicator of exposure, effects and susceptibility of the organism to the exposure of BaP (PAHs).

摘要

粗煤焦油（CCT）含有多环芳烃（PAHs）。苯并[a]芘（BaP）代谢生成一种高活性代谢物苯并[a]芘-7,8-二醇-9,10-环氧化物（BPDE），它能够与DNA结合并形成BPDE-DNA加合物。加合的DNA具有免疫原性，并通过产生针对BPDE-DNA加合物的抗体（Ab-BPDE-DNA）诱导免疫反应。循环中的Ab-BPDE-DNA被认为是BaP（PAHs）遗传毒性暴露的潜在生物标志物。银屑病的Goeckerman疗法（GT）采用皮肤涂抹CCT软膏（PAHs）。在本研究（接受GT治疗的银屑病儿童；n = 19）中，该疗法显著提高了Ab-BPDE-DNA水平（EI = 0.29/0.19 - 0.34对0.31/0.25 - 0.40；中位数/下四分位数-上四分位数；p < 0.01）。结果支持将Ab-BPDE-DNA水平作为生物体暴露于BaP（PAHs）的暴露、效应和易感性的可能初步指标的观点。